Human LDL R APC-conjugated Antibody

  (3 citations)     
Datasheet / CoA / SDS
Product Details
Citations (3)
Supplemental Products
  • Species Reactivity
  • Specificity
    Detects human LDL R in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDL R, recombinant human LRP-5, or rmLRP-6 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 472413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human LDL R
    Accession # P01130
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of LDL R in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDL R APC-conjugated Monoclonal Antibody (Catalog # FAB2148A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LDL R

The Low Density Lipoprotein Receptor (LDL R) is the founding member of the LDL R family of scavenger receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2). Human LDL R is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120-160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1). The extracellular region of human LDL R is 51% aa identical to the extracellular region of human VLDL R, and 79% aa identical to the extracellular region of mouse LDL R. LDL R is constitutively expressed and binds ApoB of LDL and ApoE of VLDL (5). It is responsible for clearing 70% of plasma LDL in liver (5). Mutations in the LDL R gene cause the autosomal dominant disorder, familial hypercholesterolemia (6).

  • References:
    1. Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
    2. Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
    3. Yamamoto, T. et al. (1984) Cell 39:27.
    4. Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828.
    5. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
    6. Varret, M. et al. (2008) Clin Genet. 73:1.
  • Long Name:
    Low Density Lipoprotein Receptor
  • Entrez Gene IDs:
    3949 (Human); 16835 (Mouse); 300438 (Rat)
  • Alternate Names:
    FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Sample Type
  1. Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9.
    Authors: Le Q, Blanchet M, Seidah N, Labonte P
    J Biol Chem, 2015;290(38):23385-400.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.
    Authors: Giugliano S, Petroff M, Warren B, Jasti S, Linscheid C, Ward A, Kramer A, Dobrinskikh E, Sheiko M, Gale M, Golden-Mason L, Winn V, Rosen H
    J Immunol, 2015;195(8):3737-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Enrichment of human hematopoietic stem/progenitor cells facilitates transduction for stem cell gene therapy.
    Authors: Baldwin K, Urbinati F, Romero Z, Campo-Fernandez B, Kaufman M, Cooper A, Masiuk K, Hollis R, Kohn D
    Stem Cells, 2015;33(5):1532-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow


No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 11  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human LDL R APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order


Customer Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Panels For

Learn More
Monoclonal Antibodies
Associated Pathways
R&D Systems Guarantee