Human LDL R PerCP-conjugated Antibody

FAB2148C has been discontinued. View all LDL R products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects human LDL R in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDL R, recombinant human LRP-5, or rmLRP-6 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 472413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human LDL R
    Accession # P01130
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of LDL R in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDL R PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2148C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LDL R

The Low Density Lipoprotein Receptor (LDL R) is the founding member of the LDL R family of receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Human LDL R is synthesized as an 860 amino acid (aa) type I transmembrane precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand binding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120-160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1, 2). The extracellular region of human LDL R shows 51% aa sequence identity to the extracellular region of human VLDL R, and 79% aa sequence identity to the extracellular region of mouse LDL R. LDL R is ubiquitously expressed, binds ApoB of LDL and ApoE of VLDL, and is responsible for clearing 70% of plasma LDL in liver (1, 5). It also is reported to bind to Factor VIII, PCSK9, and the human rhinovirus serotype 2 (1, 2, 7). Upon LDL particle binding to the hepatic LDL R, HMGCR expression is suppressed, reducing cholesterol biosynthesis; ACAT activity is increased, reducing toxic free cholesterol; and LDL R synthesis is curtailed, thereby reducing LDL uptake (1).

  • References:
    1. Go, G. and A. Mani (2012) Yale J. Biol. Med. 85:19.
    2. Blacklow, S.C. (2007) Curr. Opin. Struct. Biol. 17:419.
    3. Yamamoto, T. et al. (1984) Cell 39:27.
    4. Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828.
    5. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
    6. Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
    7. Kurasawa, J.H. et al. (2013) J. Biol. Chem. 288:22033.
  • Long Name:
    Low Density Lipoprotein Receptor
  • Entrez Gene IDs:
    3949 (Human); 16835 (Mouse); 300438 (Rat)
  • Alternate Names:
    FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor
Related Research Areas


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