Human LDL R PerCP-conjugated Antibody

FAB2148C has been discontinued. View all LDL R products.
    
Datasheet / CoA / SDS
Product Details
FAQs
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human LDL R in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDL R, recombinant human LRP-5, or rmLRP-6 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 472413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human LDL R
    Ala22-Arg788
    Accession # P01130
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of LDL R in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDL R PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2148C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LDL R

The Low Density Lipoprotein Receptor (LDL R) is the founding member of the LDL R family of receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Human LDL R is synthesized as an 860 amino acid (aa) type I transmembrane precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand binding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120-160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1, 2). The extracellular region of human LDL R shows 51% aa sequence identity to the extracellular region of human VLDL R, and 79% aa sequence identity to the extracellular region of mouse LDL R. LDL R is ubiquitously expressed, binds ApoB of LDL and ApoE of VLDL, and is responsible for clearing 70% of plasma LDL in liver (1, 5). It also is reported to bind to Factor VIII, PCSK9, and the human rhinovirus serotype 2 (1, 2, 7). Upon LDL particle binding to the hepatic LDL R, HMGCR expression is suppressed, reducing cholesterol biosynthesis; ACAT activity is increased, reducing toxic free cholesterol; and LDL R synthesis is curtailed, thereby reducing LDL uptake (1).

  • References:
    1. Go, G. and A. Mani (2012) Yale J. Biol. Med. 85:19.
    2. Blacklow, S.C. (2007) Curr. Opin. Struct. Biol. 17:419.
    3. Yamamoto, T. et al. (1984) Cell 39:27.
    4. Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828.
    5. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
    6. Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
    7. Kurasawa, J.H. et al. (2013) J. Biol. Chem. 288:22033.
  • Long Name:
    Low Density Lipoprotein Receptor
  • Entrez Gene IDs:
    3949 (Human); 16835 (Mouse); 300438 (Rat)
  • Alternate Names:
    FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Human LDL R PerCP-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order
reload

Feedback

Customer Information
Related Products
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
Associated Pathways
R&D Systems Guarantee