Human Legumain/Asparaginyl Endopeptidase Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Legumain/Asparaginyl Endopeptidase in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Legumain/Asparaginyl Endopeptidase
    Ile18-Tyr433
    Accession # Q99538
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Legumain/
Asparaginyl Endopeptidase by Western Blot.
Western blot shows lysates of human kidney tissue, human heart tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Pro-Legumain/
Asparaginyl Endopeptidase at approximately 56 kDa and mature Legumain/Asparaginyl Endopeptidase 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Legumain/Asparaginyl Endopeptidase

Legumain is a lysosomal cysteine protease whose activity is found in several tissues tested (1, 2). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (3). Over-expression of Legumain in tumors is significant for invasion/metastasis (4). Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of the cleavage, which result in the removal of the pro peptides in both C- and N-termini (5). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (6‑8). Human Legumain is synthesized as a 433 amino acid precursor with a signal peptide (residues 1‑17). The pro enzyme (residues 18‑433) was expressed with an N-terminal His tag. This activity of Legumain can be inhibited by recombinant human (rh) Cystatin C and rhCystatin E/M and recombinant mouse Cystatin C (R&D Systems, Catalog # 1196‑PI, 1286-PI and 1238-PI, respectively).

  • References:
    1. Chen, J.-M. et al. (1997) J. Biol. Chem. 272:8090.
    2. Tanaka, T. et al. (1996) Cytogenet. Cell Genet. 74:120.
    3. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
    4. Liu, C. et al. (2003) Cancer Res. 63: 2957.
    5. Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
    6. Chen, J.M. et al. (2001) Biol. Chem. 382:777.
    7. Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
    8. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.
  • Entrez Gene IDs:
    5641 (Human); 19141 (Mouse)
  • Alternate Names:
    AEP; Asparaginyl Endopeptidase; cysteine protease 1; Legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1EC 3.4.22.34
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Suppression of asparaginyl endopeptidase attenuates breast cancer-induced bone pain through inhibition of neurotrophin receptors
    Authors: P Yao, Y Ding, Z Han, Y Mu, T Hong, Y Zhu, H Li
    Mol Pain, 2017;13(0):1.74481E+15.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. The Agaricus blazei-Based Mushroom Extract, Andosan�, Protects against Intestinal Tumorigenesis in the A/J Min/+ Mouse
    PLoS ONE, 2016;11(12):e0167754.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Simvastatin inhibits glucose metabolism and legumain activity in human myotubes.
    Authors: Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H
    PLoS ONE, 2014;9(1):e85721.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish.
    Authors: Ma, Liping, Shen, Yan-Qin, Khatri, Harsh P, Schachner, Melitta
    PLoS ONE, 2014;9(4):e95098.
    Species: Zebrafish
    Sample Type: Whole Tissue
    Application: IHC Fresh
  5. Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis.
    Cell Res., 2012;22(9):1356-73.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Cystatin E/M suppresses legumain activity and invasion of human melanoma.
    Authors: Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad O, Maelandsmo GM, Solberg R
    BMC Cancer, 2010;10(1):17.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
    Authors: Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD
    Atherosclerosis, 2008;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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