Human Lgr4/GPR48 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Lgr4/GPR48 in Lgr4-transfected cell-based ELISA. Stains human Lgr4/GPR48 transfected but not irrelevant transfected cells in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 852229
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Lgr4/GPR48
    Accession # Q9BXB1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Lgr4/GPR48 in HEK293 Human Cell Line Transfected with Human Lgr4/GPR48 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48 and eGFP was stained with either (A) Mouse Anti-Human Lgr4/GPR48 APC‑conjugated Monoclonal Antibody (Catalog # FAB7750A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Lgr4/GPR48

Lgr4 (Leucine-rich repeat GPR 4), also known as GPR48 (G-Protein-coupled Receptor 48), is a seven-transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and hCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G-protein-independent mediators of the potentiating effect of R-Spondins on Wnt signaling (1-6). Lgr4 binds and forms complexes with R-Spondins, Frizzled Wnt receptors, and LRP Wnt co-receptors (5). It acts, at least in part, by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta -catenin (3, 4). Human Lgr4 cDNA encodes 951 amino acids (aa), including a long N-terminal Extracellular Domain (ECD, aa 25-544) with 16-17 LRR domains that mediate ligand interaction (1). The LRR-containing ECD of human Lgr4 shares 93% aa sequence identity with mouse, rat and bovine Lgr4, and 50-60% aa identity with human Lgr5 and Lgr6. Lgr4 is widely expressed in both embryo and adult. Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). In rodents, embryonic and adult expression includes liver, kidney, adrenals, bone/cartilage, and heart (2, 7-9). Lgr4 deletion in the mouse affects development in areas of normal expression, for example, it will inhibit fetal liver definitive erythropoiesis (9). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling (5, 6). Lgr4 may be over-expressed in carcinomas and may promote invasiveness and metastasis by down-regulating p27Kip1 expression (10).

  • References:
    1. Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.
    2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
    3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.
    4. Glinka, A. et al. (2011) EMBO Rep. 12:1055.
    5. de Lau, W. et al. (2011) Nature 476:293.
    6. Ruffner, H. et al. (2012) PLoS ONE 7:e40975.
    7. Van Schoore, G. et al. (2005) Histochem. Cell Biol. 124:35.
    8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:2241.
    9. Song, H. et al. (2008) J. Biol. Chem. 283:36687.
    10. Gao, Y. et al. (2006) Cancer Res. 66:11623.
  • Long Name:
    Leucine-rich Repeat Containing G Protein-coupled Receptor 4
  • Entrez Gene IDs:
    55366 (Human); 107515 (Mouse); 286994 (Rat)
  • Alternate Names:
    GPR48; GPR48G protein-coupled receptor 48; G-protein coupled receptor 48; leucine-rich repeat containing G protein-coupled receptor 4
Related Research Areas
Isotype Controls
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Mouse IgG2B APC-conjugated Antibody

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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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