Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cyomtetry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 707042
View all Lgr5/GPR49 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49
Met1-Ile560
Accession # O75473

Specificity

Detects human Lgr5/GPR49 in ELISAs. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in the C-terminal LRR cap of Lgr5 (Ref. 1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Lgr5/GPR49 APC‑conjugated Antibody

Detection of Lgr5/GPR49 antibody in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP antibody by Flow Cytometry.

Detection of Lgr5/GPR49 in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry.

NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with either (A) Mouse Anti-Human Lgr5/GPR49 APC-conjugated Monoclonal Antibody (Catalog # FAB8078A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Lgr5/GPR49 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Lgr5/GPR49

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal Lgr5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).

References

  1. Peng et al. (2013) Cell Rep. 3(6):1885.
  2. de Lau et al. (2014) Genes Dev. 28(4):305.
  3. Barker et al. (2013) Development 140(12):2484.
  4. Clevers (2013) Nature 495(7439):53.
  5. Wu et al. (2014) Nat Commun 5:3149.
  6. Jang et al. (2013) PLoS One 8(12): e82390495.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Gene Symbol

LGR5

UniProt

O75473 (Human)

Additional Lgr5/GPR49 Products

Product Documents for Human Lgr5/GPR49 APC‑conjugated Antibody

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Product Specific Notices for Human Lgr5/GPR49 APC‑conjugated Antibody

For research use only

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