Human Lgr5/GPR49 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Lgr5/GPR49 in ELISAs. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in the C-terminal LRR cap of Lgr5 (Ref. 1).
  • Source
    Monoclonal Mouse IgG2A Clone # 707042
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49
    Accession # O75473
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Lgr5/GPR49 in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with either (A) Mouse Anti-Human Lgr5/GPR49 APC‑conjugated Monoclonal Antibody (Catalog # FAB8078A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Lgr5/GPR49
Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal Lgr5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).
  • References:
    1. Peng et al. (2013) Cell Rep. 3(6):1885.
    2. de Lau et al. (2014) Genes Dev. 28(4):305.
    3. Barker et al. (2013) Development 140(12):2484.
    4. Clevers (2013) Nature 495(7439):53.
    5. Wu et al. (2014) Nat Commun 5:3149.
    6. Jang et al. (2013) PLoS One 8(12): e82390495.
  • Long Name:
    Leucine-rich Repeat Containing G Protein-coupled Receptor 5
  • Entrez Gene IDs:
    8549 (Human); 14160 (Mouse); 299802 (Rat)
  • Alternate Names:
    FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38
Related Research Areas
Isotype Controls
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Mouse IgG2A APC-conjugated Antibody

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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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