Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Agonist Activity, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 707042
View all Lgr5/GPR49 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49
Met1-Ile560
Accession # O75473

Specificity

Detects human Lgr5/GPR49 in ELISA. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Lgr5/GPR49 Antibody

Detection of Lgr5/GPR49 antibody in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP antibody by Flow Cytometry.

Detection of Lgr5/GPR49 in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry.

NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Lgr5/GPR49 antibody in NSO Mouse Cell Line Transfected with Human Lgr5/GPR49 by Immunocytochemistry (ICC).

Lgr5/GPR49 in NSO Mouse Cell Line Transfected with Human Lgr5/GPR49.

Lgr5/GPR49 was detected in immersion fixed NSO mouse myeloma cell line transfected with GFP (green) tagged human LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Lgr5/GPR49 antibody in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 by Immunocytochemistry (ICC).

Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49.

Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Lgr5/GPR49 antibody in Human Cells Transfected with Human Lgr5/GPR49 by Immunocytochemistry (ICC).

Lgr5/GPR49 in Human Cells Transfected with Human Lgr5/GPR49.

Lgr5/GPR49 was detected in MYC tagged human transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm.Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.
Lgr5/GPR49 antibody in Mouse Cells Transfected with Mouse Lgr5/GPR49 by Immunocytochemistry (ICC).

Lgr5/GPR49 in Mouse Cells Transfected with Mouse Lgr5/GPR49.

Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm.Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.
Human Lgr5/GPR49 Antibody Induces Activity.

Human Lgr5/GPR49 Antibody Induces Activity.

Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of R-Spondins. (Data courtesy of Dr. Wim de Lau and Dr. Hans Clevers, Hubrecht Institute, The Netherlands. See Reference 1.)

Applications for Human Lgr5/GPR49 Antibody

Application
Recommended Usage

Agonist Activity

Peng, W.C., et al. (2013) Cell Reports. 3:1885.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with GFP tagged mouse LGR5, and immersion fixed NS0 mouse myeloma cell line transfected with GFP tagged human LGR5

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lgr5/GPR49

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal LGR5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).

References

  1. Peng et al. (2013) Cell Rep. 3(6):1885.
  2. de Lau et al. (2014) Genes Dev. 28(4):305.
  3. Barker et al. (2013) Development 140(12):2484.
  4. Clevers (2013) Nature 495(7439):53.
  5. Wu et al. (2014) Nat Commun 5:3149.
  6. Jang et al. (2013) PLoS One 8(12): e82390495.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Gene Symbol

LGR5

UniProt

O75473 (Human)

Additional Lgr5/GPR49 Products

Product Documents for Human Lgr5/GPR49 Antibody

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Product Specific Notices for Human Lgr5/GPR49 Antibody

For research use only

Citations for Human Lgr5/GPR49 Antibody

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Protocols

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