Detection of Human LH beta by Western Blot.
Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LH beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8016) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LH beta at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LH beta in Human Pituitary Adenoma.
LH beta was detected in immersion fixed paraffin-embedded sections of human pituitary adenoma using Sheep Anti-Human LH beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8016) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of pituitary cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LH beta
LH beta (Luteinizing Hormone subunit beta; also LSH-B and lutropin beta chain) is a 17-19 kDa member of the glycoprotein hormone subunit beta family, cysteine knot superfamily of proteins. It is synthesized by gonadotrophs in the anterior pituitary, and noncovalently heterodimerizes with the 21-23 kDa common glycoprotein alpha chain, forming 40-42 kDa LH. LH has effects on at least three organ systems. In the ovary, LH induces the synthesis of testosterone, which is quickly converted to estradiol. Estradiol subsequently induces a bolus release of LH from the pituitary, resulting in ovulation. LH now drives the formation of the corpus luteum (or yellow body, from luteus meaning yellow) with the production of progesterone. In the testis, LH induces Leydig cell production of testosterone that activates genes in Sertoli cells drives sperm formation. And in the adrenal, LH appears to induce secretion of sulfated DHEA, a precursor to androgens. Mature human LH beta is 121 amino acids (aa) in length (aa 21-141). It contains three intrachain disulfide bonds that form a cysteine knot, plus one utilized N-linked glycosylation site at Asn50 that incorporates both fucose and sulfate on galactose. The presence of carbohydrate is necessary for bioactivity. Multiple isoforms of LH exist, and likely represent variations in carbohydrate structure. Full-length human LH beta shares 82% and 72% aa sequence identity between human chorionic gonadotrophin/CG beta and mouse LH beta subunits, respectively.
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