Human LIF Quantikine ELISA Kit

Human LIF ELISA Standard Curve
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Citations (18)
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Human LIF Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
5.36 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human LIF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human LIF Immunoassay is a 4.5 hours solid phase ELISA designed to measure human LIF in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human LIF and antibodies raised against the recombinant protein. Results obtained for naturally occurring human LIF showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human LIF.

Precision

Intra-Assay Precision (Precision within an assay) <div>Three samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) <div>Three samples of known concentration were tested in twenty separate assays to assess&nbsp;inter-assay precision. Assays were performed by at least three technicians using two lots of components.</div>

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 255 778 1433 268 827 1548
Standard Deviation 11 21.8 32.6 21 50.9 76.7
CV% 4.3 2.8 2.3 7.8 6.2 5

Recovery

The recovery of human LIF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 92-112
EDTA Plasma (n=4) 90 83-96
Heparin Plasma (n=4) 90 83-99
Serum (n=4) 92 83-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human LIF were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. 
Human LIF ELISA Linearity

Data Examples

Human LIF ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LIF

Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.

Long Name:
Leukemia Inhibitory Factor
Entrez Gene IDs:
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names:
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

Citations for Human LIF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. LIFR increases the release of sEng via the up-regulation of MMP14 expression in PE
    Authors: H Li, J Yao, X Chang, J Wu, T Duan, K Wang
    Reproduction, 2018;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  2. The role of p38 mitogen-activated protein kinase in serum-induced leukemia inhibitory factor secretion by bone marrow stromal cells from pediatric myelodysplastic syndromes.
    Authors: da Costa SV, Roela RA, Junqueira MS, Arantes C, Brentani MM
    Leuk. Res., 2010;34(4):507-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of patients with adenomyosis during implantation window.
    Authors: Xiao Y, Sun X, Yang X, Zhang J, Xue Q, Cai B, Zhou Y
    Fertil. Steril., 2010;94(1):85-9.
    Species: Human
    Sample Types: Uterine Lavage Fluid
  4. Prokineticin 1 mediates fetal-maternal dialogue regulating endometrial leukemia inhibitory factor.
    Authors: Evans J, Catalano RD, Brown P, Sherwin R, Critchley HO, Fazleabas AT, Jabbour HN
    FASEB J., 2009;23(7):2165-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Helicobacter pylori cytotoxin-associated gene A activates the signal transducer and activator of transcription 3 pathway in vitro and in vivo.
    Authors: Bronte-Tinkew DM, Terebiznik M, Franco A, Ang M, Ahn D, Mimuro H, Sasakawa C, Ropeleski MJ, Peek RM, Jones NL
    Cancer Res., 2009;69(2):632-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Isoflavones regulate secretion of leukemia inhibitory factor and transforming growth factor {beta} and expression of glycodelin in human endometrial epithelial cells.
    Authors: Xu JW, Yasui N, Ikeda K, Pan WJ, Watanabe J, Shiotani M, Yanaihara A, Miki T, Yamori Y
    J. Endocrinol., 2008;196(2):425-33.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression.
    Authors: Kamohara H, Ogawa M, Ishiko T, Sakamoto K, Baba H
    Int. J. Oncol., 2007;30(4):977-83.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
    Authors: Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR
    J. Neurosci., 2006;26(47):12174-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
  11. Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure.
    Authors: Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K
    Heart Vessels, 2004;19(5):237-41.
    Species: Human
    Sample Types: Plasma
  12. Role of mitogen-activated protein kinase family in serum-induced leukaemia inhibitory factor and interleukin-6 secretion by bone marrow stromal cells.
    Authors: Nakao T, Kim S, Ohta K, Kawano H, Hino M, Miura K, Tatsumi N, Iwao H
    Br. J. Pharmacol., 2002;136(7):975-84.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx.
    Authors: Chen CD, Yang JH, Lin KC, Chao KH, Ho HN, Yang YS
    Hum. Reprod., 2002;17(1):128-33.
    Species: Human
    Sample Types: Hydrosalpinx Fluid
  14. Effect of azacytidine in the release of leukemia inhibitory factor, oncostatin m, interleukin (IL)-6, and IL-11 by mononuclear cells of patients with refractory anemia.
    Authors: Lopez-Karpovitch X, Barrales-Benitez O, Flores M, Piedras J
    Cytokine, 2002;20(4):154-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
    Authors: Carstanjen D, Regenfus M, Muller C, Salama A
    Exp. Hematol., 2001;29(7):812-21.
    Species: Human
    Sample Types: Plasma
  16. Expression of interleukin-6, leukemia inhibitory factor and their receptors by colonic epithelium and pericryptal fibroblasts.
    Authors: Rockman SP, Demmler K, Roczo N, Cosgriff A, Phillips WA, Thomas RJ, Whitehead RH
    J. Gastroenterol. Hepatol., 2001;16(9):991-1000.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Distinct hematopoietic support by two human stromal cell lines.
    Authors: Loeuillet C, Bernard G, Remy-Martin J, Saas P, Herve P, Douay L, Chalmers D
    Exp. Hematol., 2001;29(6):736-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Influence of protein supplements on the secretion of leukaemia inhibitory factor by mitomycin-pretreated Vero cells: possible application to the in vitro production of bovine blastocysts with high cryotolerance.
    Authors: Carnegie JA, Morgan JJ, McDiarmid N, Durnford R
    J. Reprod. Fertil., 1999;117(1):41-8.
    Species: Primate - Cholrocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Culture Supernates

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