Human LILRA6/CD85b Antibody

Catalog # Availability Size / Price Qty
MAB8656
MAB8656-SP
Detection of LILRA6/CD85b in HEK293 Human Cell Line Transfected with Human LILRA6/CD85b and eGFP by Flow Cytometry.
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Human LILRA6/CD85b Antibody Summary

Species Reactivity
Human
Specificity
Detects human LILRA6/CD85b in direct ELISA. Detects HEK293 cells transfected with LILRA6/CD85b but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 921330
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line-derived recombinant human LILRA6/CD85b
Met1-Asn447
Accession # Q6PI73
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of LILRA6/CD85b in HEK293 Human Cell Line Transfected with Human LILRA6/CD85b and eGFP by Flow Cytometry. View Larger

Detection of LILRA6/CD85b in HEK293 Human Cell Line Transfected with Human LILRA6/CD85b and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human LILRA6/CD85b and eGFP were stained with either (A) Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Flow Cytometry Detection of LILRA6/CD85b in Human PBMCs by Flow Cytometry. View Larger

Detection of LILRA6/CD85b in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA6/CD85b

LILRA6 (Leukocyte immunoglobulin-like receptor subfamily A, member 6) is also known as ILT5 and CD85b.   The leukocyte immunoglobulin-like receptors (LILR) are a family of receptors possessing extracellular immunoglobulin domains. They are also known as ILTs and can exert immunomodulatory effects on a wide range of immune cells. LILRA6 is a highly polymorphic surface molecule that is one of the targets of dendritic cell-reactive antibodies.

Long Name
Leukocyte Immunoglobulin-like Receptor A6
Entrez Gene IDs
79168 (Human); 18726 (Mouse)
Alternate Names
7M1; CD85b; ILT8; LILRA6; Pira3

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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