Human LILRB4/CD85k/ILT3 Antibody [Biotin]

R&D Systems | Catalog # BAF2425

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Western Blot, Flow Cytometry, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all LILRB4/CD85k/ILT3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRB4/CD85k/ILT3
Pro17-His257
Accession # ABM86208

Specificity

Detects human LILRB4/CD85k/ILT3 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ILT4 and rhILT5 and 5% cross-reactivity with rhILT2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LILRB4/CD85k/ILT3 Antibody [Biotin]

Detection of Human ILT3/CD85k/LILRB4 by Flow Cytometry

Detection of Human ILT3/CD85k/LILRB4 by Flow Cytometry

CSF monocytes express high levels of ILT3.(A) Paired blood and CSF samples were analyzed for expression of ILT3 on CD14+ monocytes by flow cytometry. The specific fluorescent index is shown. Patients were categorized according to their diagnosis as having non-inflammatory neurological diseases (NIND, n = 14; marked by dot) or inflammatory neurological disease (IND, n = 8) including 5 MS patients (marked by x) and 3 patients with other inflammatory neurological disease (diamond). A Mann-Whitney U test was performed to assess statistical significance. (B) Histogram of a representative staining in a patient with NIND. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25551576), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human ILT3/CD85k/LILRB4 by Flow Cytometry

Detection of Human ILT3/CD85k/LILRB4 by Flow Cytometry

CSF monocytes express high levels of ILT3.(A) Paired blood and CSF samples were analyzed for expression of ILT3 on CD14+ monocytes by flow cytometry. The specific fluorescent index is shown. Patients were categorized according to their diagnosis as having non-inflammatory neurological diseases (NIND, n = 14; marked by dot) or inflammatory neurological disease (IND, n = 8) including 5 MS patients (marked by x) and 3 patients with other inflammatory neurological disease (diamond). A Mann-Whitney U test was performed to assess statistical significance. (B) Histogram of a representative staining in a patient with NIND. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25551576), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LILRB4/CD85k/ILT3 Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human LILRB4/CD85k/ILT3

Reviewed Applications

Read 1 review rated 4 using BAF2425 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB4/CD85k/ILT3

ILT3, also known as leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) and leukocyte immunoglobulin-like receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4

Alternate Names

CD85k, HM18, ILT3, LIR5

Entrez Gene IDs

11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)

Gene Symbol

LILRB4

UniProt

ABM86208

Additional LILRB4/CD85k/ILT3 Products

Product Documents for Human LILRB4/CD85k/ILT3 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LILRB4/CD85k/ILT3 Antibody [Biotin]

For research use only

Citations for Human LILRB4/CD85k/ILT3 Antibody [Biotin]

Customer Reviews for Human LILRB4/CD85k/ILT3 Antibody [Biotin] (1)

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  • Human LILRB4/CD85k/ILT3 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/07/2020
    Human LILRB4/CD85k/ILT3 Antibody [Biotin] BAF2425

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