Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Lipocalin-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lipocalin-1 (R&D Systems, Catalog # 1708-PI)
His19-Asp176
Accession # P31025

Specificity

Detects human Lipocalin-1 in Western blots. In this format, approximately 5% cross‑reactivity with rhLipocalin-2 and rmLipocalin-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Lipocalin‑1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Lipocalin‑1 (Catalog # 1708-PI)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lipocalin-1

Lipocalin-1, also known as tear prealbumin or von Ebner’s gland protein (VEGP), is encoded by the LCN1 gene (1 - 3). It is a member of the Lipocalin superfamily that binds many different classes of lipophylic chemicals (4). Lipocalin-1 contains three sequence motifs similar to the cystatins, a superfamily of cysteine protease inhibitors (5). In fact, it has been suggested that Lipocalin-1 is a physiological inhibitor of cysteine proteases and plays a role in the control of inflammatory processes in oral and ocular tissues (5). rhLipocalin-1 corresponds to the mature and secreted protein. It is a weak inhibitor of cysteine proteases such as cathepsin V, which is similar to rhCystatin S.

References

  1. Redl, B. et al. (1992) J. Biol. Chem. 267:20282.
  2. Blaker, M. et al. (1993) Biochim. Biophys. Acta 1172:131.
  3. Lassagne, H. and A.M. Gachon (1993) Exp. Eye Res. 56:605.
  4. Redl, B. et al. (2000) Biochim. Biophys. Acta 1482:241.
  5. van’t Hof, W. et al. (1997) J. Biol. Chem. 272:1837.

Alternate Names

LCN1, Lipocalin1, Tear Prealbumin, VEGP

Entrez Gene IDs

3933 (Human)

Gene Symbol

LCN1

UniProt

P31025

Additional Lipocalin-1 Products

Product Documents for Human Lipocalin‑1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Lipocalin‑1 Biotinylated Antibody

For research use only

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