Human LRIG1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7498P-025
Detection of LRIG1 in LNCaP Human Cell Line by Flow Cytometry.
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Human LRIG1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRIG1 in ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse LRIG1 and recombinant human LRIG3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 789211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human LRIG1
Ala35-Ser779
Accession # Q96JA1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of LRIG1 in LNCaP Human Cell Line by Flow Cytometry. View Larger

Detection of LRIG1 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human LRIG1 PE‑conjugated Monoclonal Antibody (Catalog # FAB7498P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LRIG1

LRIG1 (Leucine-rich Repeats and Ig-like domains-1), also known as LIG-1, is an approximately 134-145 kDa type I transmembrane glycoprotein that belongs to the LRIG gene family. It is widely expressed and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle cells, mammary epithelium, cardiac muscle cells, keratinocytes and neurons. LRIG1 is believed to negatively regulate the ErbB family of receptors. In particular, and in a ligand-independent manner, LRIG1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number. Alternatively, LRIG1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction. Mature human LRIG1 is 1059 amino acids (aa) in length. It contains a large 760 amino acid (aa) extracellular domain (ECD) (aa 35-794) plus a 278 aa cytoplasmic region. The ECD contains 17 LRRs (aa 35‑491) and three C2-type Ig-like domains (aa 495-780). These two domain types are each sufficient for EGFR binding. There are two potential alternative splice forms. One contains a 27 aa insertion after Gly874, while another shows a 24 aa insertion after Lys387 coupled to a Gln substitution for aa 644-691. The LRIG1 ECD undergoes proteolysis, generating 100-110 and 55-60 kDa soluble fragments. Over aa 35‑779, human LRIG1 shares 90% aa sequence identity with mouse LRIG1.

Long Name
Leucine-rich Repeats and Immunoglobulin-like Domains 1
Entrez Gene IDs
26018 (Human); 16206 (Mouse); 312574 (Rat)
Alternate Names
D6Bwg0781e; Img; LIG1; LIG-1; LRIG1

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