Human LRIG1 Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human LRIG1 in ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse LRIG1 and recombinant human LRIG3 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 789211
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with human LRIG1
    Ala35-Ser779
    Accession # Q96JA1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LRIG1 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Immunocytochemistry
LRIG1 in SK‑BR‑3 Human Cell Line. LRIG1 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
LRIG1 in Human Kidney. LRIG1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRIG1

LRIG1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 134-145 kDa glycoprotein that belongs to the LRIG gene family. It is widely expressed and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons. LRIG1 is believed to negatively regulate the ErbB family of receptors. In particular, and in a ligand-independent manner, LRIG1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number. Alternatively, LRIG1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction. Mature human LRIG1 is a 1059 amino acid (aa) type I transmembrane protein. It contains a large 760 amino acid (aa) extracellular domain (ECD) (aa 35-794) plus a 278 aa cytoplasmic region. The ECD contains 17 LRRs (aa 35‑491) and three C2-type Ig-like domains (aa 495-780). These two domain types are each sufficient for EGFR binding. There are two potential alternative splice forms. One contains a 27 aa insertion after Gly874, while another shows a 24 aa insertion after Lys387 coupled to a Gln substitution for aa 644-691. The LRIG1 ECD undergoes proteolysis, generating 100-110 and 55-60 kDa soluble fragments. Over aa 35‑779, human LRIG1 shares 90% aa sequence identity with mouse LRIG1.

  • Long Name:
    Leucine-rich Repeats and Immunoglobulin-like Domains 1
  • Entrez Gene IDs:
    26018 (Human); 16206 (Mouse); 312574 (Rat)
  • Alternate Names:
    D6Bwg0781e; Img; LIG1; LIG-1; LRIG1
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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 Flow Human Anonymous 06/28/2016
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Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood lymphocytes (PBL)
SpeciesHuman

Other Experimental Details

Other Experimental DetailsUsed with anti-mouse secondary in PE channel for detection. Could not see shift above background staining. One published report of expression on cell type, trying to replicate. May be antigen is not expressed rather than antibody poor as we have no real control.

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