Human LRIG1 Antibody

Catalog # Availability Size / Price Qty
MAB7498
MAB7498-SP
Detection of LRIG1 in LNCaP Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human LRIG1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRIG1 in ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse LRIG1 and recombinant human LRIG3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 789211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human LRIG1
Ala35-Ser779
Accession # Q96JA1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LRIG1 antibody in LNCaP Human Cell Line antibody by Flow Cytometry. View Larger

Detection of LRIG1 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunocytochemistry LRIG1 antibody in SK-BR-3 Human Cell Line by Immunocytochemistry (ICC). View Larger

LRIG1 in SK‑BR‑3 Human Cell Line. LRIG1 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry LRIG1 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

LRIG1 in Human Kidney. LRIG1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRIG1

LRIG1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 134-145 kDa glycoprotein that belongs to the LRIG gene family. It is widely expressed and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons. LRIG1 is believed to negatively regulate the ErbB family of receptors. In particular, and in a ligand-independent manner, LRIG1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number. Alternatively, LRIG1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction. Mature human LRIG1 is a 1059 amino acid (aa) type I transmembrane protein. It contains a large 760 amino acid (aa) extracellular domain (ECD) (aa 35-794) plus a 278 aa cytoplasmic region. The ECD contains 17 LRRs (aa 35‑491) and three C2-type Ig-like domains (aa 495-780). These two domain types are each sufficient for EGFR binding. There are two potential alternative splice forms. One contains a 27 aa insertion after Gly874, while another shows a 24 aa insertion after Lys387 coupled to a Gln substitution for aa 644-691. The LRIG1 ECD undergoes proteolysis, generating 100-110 and 55-60 kDa soluble fragments. Over aa 35‑779, human LRIG1 shares 90% aa sequence identity with mouse LRIG1.

Long Name
Leucine-rich Repeats and Immunoglobulin-like Domains 1
Entrez Gene IDs
26018 (Human); 16206 (Mouse); 312574 (Rat)
Alternate Names
D6Bwg0781e; Img; LIG1; LIG-1; LRIG1

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Citation for Human LRIG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Contribution of GATA6 to homeostasis of the human upper pilosebaceous unit and acne pathogenesis
    Authors: B Oulès, C Philippeos, J Segal, M Tihy, M Vietri Rud, AM Cujba, PA Grange, S Quist, K Natsuga, L Deschamps, N Dupin, G Donati, FM Watt
    Nat Commun, 2020;11(1):5067.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human LRIG1 Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human LRIG1 Antibody
By Anonymous on 10/30/2021
Application: IHC Sample Tested: Gastric epithelium Species: Human

Human LRIG1 Antibody
By Anonymous on 06/28/2016
Application: Flow Sample Tested: Peripheral blood lymphocytes (PBL) Species: Human

Used with anti-mouse secondary in PE channel for detection. Could not see shift above background staining. One published report of expression on cell type, trying to replicate. May be antigen is not expressed rather than antibody poor as we have no real control.