Human LRRTM4 Antibody Summary
Accession # Q86VH4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human LRRTM4 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LRRTM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5377) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRRTM4 at approximately 65 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LRRTM4 (leucine rich repeat transmembrane protein 4) is a 59kDa (predicted) and 67 kDa (predicted) glycoprotein, member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM4 is found in the neurons of the cerebral cortex, dentate gyrus granule cells, and cerebellar Purkinje cells. Mature human LRRTM4 is 560 amino acids (aa) in length. The extracellular region contains one Cys‑rich N-terminal flanking domain (aa 33‑59), multiple LRRs (aa 60‑299), and a Cys‑rich C-terminal flanking domain (aa 306‑363). There is one splice variant that shows a Val substitution for cytoplasmic aa 518‑590. Over aa 31‑424, human LRRTM4 shares 95% aa identity with mouse LRRTM4.
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