Human LRRTM4 Antibody

Catalog # Availability Size / Price Qty
AF5377
AF5377-SP
Detection of Human LRRTM4 by Western Blot.
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Human LRRTM4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRRTM4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) LRRTM1, rhLRRTM2, and rhLRRTM3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRRTM4
Gln31-Lys424
Accession # Q86VH4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LRRTM4 by Western Blot. View Larger

Detection of Human LRRTM4 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LRRTM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5377) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRRTM4 at approximately 65 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRTM4

LRRTM4 (leucine rich repeat transmembrane protein 4) is a 59kDa (predicted) and 67 kDa (predicted) glycoprotein, member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM4 is found in the neurons of the cerebral cortex, dentate gyrus granule cells, and cerebellar Purkinje cells. Mature human LRRTM4 is 560 amino acids (aa) in length. The extracellular region contains one Cys‑rich N-terminal flanking domain (aa 33‑59), multiple LRRs (aa 60‑299), and a Cys‑rich C-terminal flanking domain (aa 306‑363). There is one splice variant that shows a Val substitution for cytoplasmic aa 518‑590. Over aa 31‑424, human LRRTM4 shares 95% aa identity with mouse LRRTM4.

Long Name
Leucine Rich Repeat Transmembrane Neuronal 4
Entrez Gene IDs
80059 (Human); 500219 (Rat)
Alternate Names
FLJ12568; leucine rich repeat transmembrane neuronal 4; leucine-rich repeat transmembrane neuronal 4 protein; leucine-rich repeat transmembrane neuronal protein 4; LRRTM4; MGC120633; MGC120636

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