Human Lymphotoxin-alpha /TNF-beta Antibody

Catalog # Availability Size / Price Qty
MAB211
MAB211-SP
Human Lymphotoxin-alpha /TNF-beta  Antibody in Neutralization
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Human Lymphotoxin-alpha /TNF-beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lymphotoxin‑ alpha /TNF‑ beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TNF-alpha is observed. Neutralizes the biological activity of recombinant human TNF-beta. Will not neutralize the biological activity of recombinant human TNF-alpha or recombinant mouse TNF-alpha.
Source
Monoclonal Mouse IgG1 Clone # 5802
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human Lymphotoxin‑ alpha /TNF‑ beta
Leu35-Leu205
Accession # P01374
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize Lymphotoxin‑ alpha /TNF‑ beta -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 75-600 ng/mL in the presence of 50 pg/mL Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta and actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Cytotoxicity Induced by Lymphotoxin‑ alpha /TNF‑ beta  and Neutral-ization by Human Lymphotoxin-alpha /TNF-beta  Antibody. View Larger

Cytotoxicity Induced by Lymphotoxin‑ alpha /TNF‑ beta and Neutral-ization by Human Lymphotoxin-alpha /TNF-beta Antibody. Recombinant Human Lymphotoxin-a/TNF-beta (Catalog # 211-TB) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Human Lymphotoxin-a/TNF-beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Lymphotoxin-a/TNF-beta Monoclonal Antibody (Catalog # MAB211). The ND50is typically 75-600 ng/mL in the presence of the metabolic inhibitor actinomycin D.

Immunocytochemistry Lymphotoxin‑ alpha /TNF‑ beta  in Human PBMCs. View Larger

Lymphotoxin‑ alpha /TNF‑ beta in Human PBMCs. Lymphotoxin-a/TNF-beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human Lymphotoxin-a/TNF-beta Monoclonal Antibody (Catalog # MAB211) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin-alpha/TNF-beta

Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex.

TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.

Long Name
Tumor Necrosis Factor beta
Entrez Gene IDs
4049 (Human); 16992 (Mouse)
Alternate Names
LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

Product Datasheets

Citation for Human Lymphotoxin-alpha /TNF-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha?
    Authors: Buch MH, Conaghan PG, Quinn MA, Bingham SJ, Veale D, Emery P
    Ann. Rheum. Dis., 2004;63(10):1344-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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