Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody

R&D Systems | Catalog # AF6746

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Lysine (K)-specific Demethylase 3A/KDM3A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KDM3A
Pro844-Glu968
Accession # Q9Y4C1

Specificity

Detects human KDM3A in direct ELISAs and Western blots. In direct ELISAs, approximately 3% cross-reactivity with recombinant human (rh) KDM4A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody

Detection of Human Lysine (K)-specific Demethylase 3A/KDM3A antibody by Western Blot.

Detection of Human Lysine (K)-specific Demethylase 3A/KDM3A by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human KDM3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6746) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KDM3A at approximately 150 kDa (as indicated), the band at approximately 70kDa represents the product of degradation or processing. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: 293T human embryonic kidney cell line and MOLT‑4 human acute lymphoblastic leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lysine (K)-specific Demethylase 3A/KDM3A

JMJD1A (Jumonji [Japanese for "cruciform/cross"]/ARID domain-containing protein 1A; also KDM3A, JHDM2A, and TSGA) is a 140-150 kDa member of the JHDM2 histone demethylase family of proteins. It is induced by hypoxia, and expressed in spermatids, smooth muscle cells, mammary epithelium, hepatocytes, and renal tubular epithelium. JMJD1A is reported to demethylate di- and mono-methylated Lys9 on histone H3. It has no activity on trimethylated Lys9. JMJD1A is both cytoplasmuc and nuclear, and serves as a coactivator for the AR. Human JMJD1A is 1321 amino acids (aa) in length. It contains one C6-type zinc-finger motif (aa 662-687) and a jumonji C type catalytic domain (aa 1151-1264). There is one phosphorylation site at Ser445. There is one potential splice variants that shows a 13 aa substitution for aa 699-1321. Over aa 810-968, human JMJD1A shares 94% aa identity with mouse JMJD1A.

Alternate Names

JHDM2A, JMJD1A, KDM3A, KIAA0742, Lysine (K)specific Demethylase 3A, TSGA

Entrez Gene IDs

55818 (Human); 104263 (Mouse); 312440 (Rat)

Gene Symbol

KDM3A

UniProt

Q9Y4C1

Additional Lysine (K)-specific Demethylase 3A/KDM3A Products

Product Documents for Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody

Certificate of Analysis

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Product Specific Notices for Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody

For research use only

Related Research Areas

Epigenetics

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