Detection of Human LYVE‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20892) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LYVE‑1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LYVE-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20892, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1 ‑ 3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9 ‑11).
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Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
Banerji, S. et al. (1999) J. Cell Biol. 144:789.
Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Showing Results 1 - 1 of 1
Lymphatic vessels in osteoarthritic human knees. Authors: Walsh D, Verghese P, Cook G, McWilliams D, Mapp P, Ashraf S, Wilson D Osteoarthritis Cartilage, 2012;20(5):405-12. Species: Human Sample Type: Whole Tissue Application: IHC Not Specified
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