Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MCEMP1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MCEMP1
Val106-Gln187
Accession # Q8IX19

Specificity

Detects human MCEMP1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human MCEMP1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood and THP‑1 human acute monocytic leukemia cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human MCEMP1

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MCEMP1

MCEMP1 (mast cell-expressed membrane protein 1; also C19orf59) is a 22 kDa mast cell specific protein expressed in lung. Human MCEMP1 is 187 amino acids (aa) in length. It is a type II transmembrane protein that contains an 85 aa N-terminal cytoplasmic region and an 81 aa C-terminal extracellular domain (aa 107‑187). There is one potential splice variant that shows a 22 aa substitution for the C-terminal five amino acids. Over aa 106‑187, human MCEMP1 shares only 52% aa identity with the presumed mouse MCEMP1 ortholog.

Long Name

Mast Cell-expressed Membrane Protein 1

Alternate Names

C19orf59

Entrez Gene IDs

199675 (Human); 69189 (Mouse); 498128 (Rat)

Gene Symbol

MCEMP1

UniProt

Q8IX19

Additional MCEMP1 Products

Product Documents for Human MCEMP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MCEMP1 Antibody

For research use only

Related Research Areas

Mast Cells

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