Human Mer Antibody [Biotin]

R&D Systems | Catalog # BAF891

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Mer Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Mer
Arg26-Ala499
Accession # Q12866

Specificity

Detects human Mer in ELISAs and Western blots. In sandwich immunoassays, approximately 6% cross-reactivity with recombinant mouse Mer is observed, and less than 0.3% cross-reactivity with recombinant human (rh) Axl is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Mer Antibody [Biotin]

Human Mer ELISA Standard Curve

Human Mer ELISA Standard Curve

Recombinant Human Mer Fc Chimera (Catalog # 891-MR) was serially diluted and captured by Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Mer Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Mer Fc Chimera (Catalog # 891-MR)

Human Mer Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Mer Antibody (Catalog # MAB8912)
  • Standard: Recombinant Human Mer Fc Chimera Protein, CF (Catalog # 891-MR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF891 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

Q12866

Additional Mer Products

Product Documents for Human Mer Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mer Antibody [Biotin]

For research use only

Citations for Human Mer Antibody [Biotin]

Customer Reviews for Human Mer Antibody [Biotin] (2)

4.5 out of 5
2 Customer Ratings
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  • Human Mer Biotinylated Antibody
    Name: Sophie Lin
    Application: ELISA
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 04/26/2017
  • Human Mer Biotinylated Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUMAN MACROPHAGES
    Species: Human
    Verified Customer | Posted 07/03/2016

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