Human Mer Antibody
Human Mer Antibody Summary
Arg26-Ala499
Accession # Q12866
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Mer by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human Mer Specificity by Using Knockout Cell Line. Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and Mer knockout HepG2 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 175 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mer
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Product Datasheets
Citations for Human Mer Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
Nature Medicine, 2022;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages
Authors: F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering
Cell Death & Disease, 2021;12(6):538.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
Virology, 2018;515(0):176-190.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human Sertoli cells support high levels of Zika virus replication and persistence
Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
Sci Rep, 2018;8(1):5477.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses
Authors: AS Richard, BS Shim, YC Kwon, R Zhang, Y Otsuka, K Schmitt, F Berri, MS Diamond, H Choe
Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):2024-2029.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
Br. J. Cancer, 2017;117(12):1787-1797.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation
Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams
J. Immunol., 2017;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
Authors: Zizzo G, Cohen P
J Immunol, 2013;190(10):5237-46.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
J Immunol, 2012;189(7):3508-20.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
J. Exp. Med., 2012;209(11):2033-47.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages.
Authors: Eken C, Martin PJ, Sadallah S, Treves S, Schaller M, Schifferli JA
J. Biol. Chem., 2010;285(51):39914-21.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
Authors: Uehara H, Shacter E
J. Immunol., 2008;180(4):2522-30.
Species: Human
Sample Types: Cell Lysates
Applications: Neutralization
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