Human MFNG Antibody

Catalog # Availability Size / Price Qty
Detection of Human MFNG by Western Blot.
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Product Details
Supplemental Products

Human MFNG Antibody Summary

Species Reactivity
Detects human MFNG in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human MFNG
Accession # O00587
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MFNG, see our available Western blot detection antibodies.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MFNG antibody by Western Blot. View Larger

Detection of Human MFNG by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human MFNG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MFNG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFNG

MFNG (Manic fringe N-acetylglucosaminyltransferase; also O-fucosylpeptide 3-beta -N-acetylglucosaminyltransferase) is a 52 - 55 kDa member of the glucosyltransferase 31 family. It is a Golgi membrane protein that transfers N-acetylglucosamine to an O-linked fucose residue on Notch. Activity on Notch increases Delta-1 induced signaling while suppressing Jagged-1 signaling. MFNG is found in fetal pancreatic endocrine progenitor cells and immature ventricular zone neurons. Human MFNG is a 321 amino acid (aa) type II transmembrane protein. It contains a short 7 aa cytoplasmic region, plus a 294 aa luminal domain (aa 28-321). There are two potential splice variants, one that shows a 15 aa substitution for aa 104-321, and another that contains a three aa substitution for aa 86-102. Over aa 37-321, human MFNG shares 85% aa identity with mouse MFNG.

Long Name
Manic Fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Entrez Gene IDs
4242 (Human)
Alternate Names
beta-1,3-N-acetylglucosaminyltransferase manic fringe; EC; manic fringe (Drosophila) homolog; manic fringe homolog (Drosophila); MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; MFNG; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

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