|Detection of Human MFNG by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human MFNG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MFNG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
MFNG (Manic fringe N-acetylglucosaminyltransferase; also O-fucosylpeptide 3-beta -N-acetylglucosaminyltransferase) is a 52 - 55 kDa member of the glucosyltransferase 31 family. It is a Golgi membrane protein that transfers N-acetylglucosamine to an O-linked fucose residue on Notch. Activity on Notch increases Delta-1 induced signaling while suppressing Jagged-1 signaling. MFNG is found in fetal pancreatic endocrine progenitor cells and immature ventricular zone neurons. Human MFNG is a 321 amino acid (aa) type II transmembrane protein. It contains a short 7 aa cytoplasmic region, plus a 294 aa luminal domain (aa 28-321). There are two potential splice variants, one that shows a 15 aa substitution for aa 104-321, and another that contains a three aa substitution for aa 86-102. Over aa 37-321, human MFNG shares 85% aa identity with mouse MFNG.
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