Human mGluR1 Antibody

R&D Systems | Catalog # AF4836

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all mGluR1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human mGluR1
Ser33-Ser522
Accession # Q13255

Specificity

Detects human mGluR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) mGluR4, rhmGluR8, rhmGluR2, rhmGluR3 and rhmGluR7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human mGluR1 Antibody

Detection of mGluR1 by Immunohistochemistry

Detection of mGluR1 by Immunohistochemistry

mGluR1 alpha expression and quantification of immunoreactivity in the hippocampus in human control and Alzheimer’s disease cases visualized by fluorescent immunohistochemistry. Photomicrographs of representative regions of the CA1(A), CA2 (B), CA3 (C), and dentate gyrus (D) showing mGluR1 alpha (red) and mGluR1 alpha overlaid with NeuN (green) immunoreactivity for representative Alzheimer’s disease and control cases. In the hippocampal CA2, CA3, and DG subfields, mGluR1 alpha density shows no statistically significant change in AD (white bars; n = 6) compared to control (black bars; n = 7) cases (Unpaired Mann-Whitney test). The figure shows a significant decrease in mGluR1 alpha expression in the stratum (str.) pyramidale (*p ≤ 0.05), and radiatum (**p ≤ 0.01) of the CA1 region in AD cases. Data is expressed as mean with individual data points representing single cases. AD = Alzheimer’s disease; CA = cornu ammonis; DG = dentate gyrus; str. ori = stratum oriens; str. pyr = stratum pyramidale; str. rad = stratum radiatum; str. mol = stratum moleculare; str. gran = stratum granulosum. Scale bars A-C= 100 µm; D = 50 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36590090), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of mGluR1 by Immunohistochemistry

Detection of mGluR1 by Immunohistochemistry

mGluR1 alpha expression and quantification of immunoreactivity in the subiculum, entorhinal cortex and superior temporal gyrus in human control and Alzheimer’s disease cases. Photomicrographs of representative regions of the subiculum (A), entorhinal cortex (B), and superior temporal gyrus (C) showing mGluR1 alpha (red) and mGluR1 alpha overlaid with NeuN (green) immunoreactivity for representative Alzheimer’s disease and control cases. In the STG, mGluR1 alpha density shows no statistically significant change in AD (white bars; n = 6) compared to control (black bars; n = 7) cases (Unpaired Mann-Whitney test). The figure shows a significant decrease in mGluR1 alpha expression in the subiculum (*p ≤ 0.05) and entorhinal cortex (*p ≤ 0.05) in AD cases. Data is expressed as mean with individual data points representing single cases. AD = Alzheimer’s disease; ECx = entorhinal cortex: STG = superior temporal gyrus. Scale bars A-C = 100 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36590090), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of mGluR1 by Immunohistochemistry

Detection of mGluR1 by Immunohistochemistry

mGluR1 alpha expression and quantification of immunoreactivity in the hippocampus in human control and Alzheimer’s disease cases visualized by fluorescent immunohistochemistry. Photomicrographs of representative regions of the CA1(A), CA2 (B), CA3 (C), and dentate gyrus (D) showing mGluR1 alpha (red) and mGluR1 alpha overlaid with NeuN (green) immunoreactivity for representative Alzheimer’s disease and control cases. In the hippocampal CA2, CA3, and DG subfields, mGluR1 alpha density shows no statistically significant change in AD (white bars; n = 6) compared to control (black bars; n = 7) cases (Unpaired Mann-Whitney test). The figure shows a significant decrease in mGluR1 alpha expression in the stratum (str.) pyramidale (*p ≤ 0.05), and radiatum (**p ≤ 0.01) of the CA1 region in AD cases. Data is expressed as mean with individual data points representing single cases. AD = Alzheimer’s disease; CA = cornu ammonis; DG = dentate gyrus; str. ori = stratum oriens; str. pyr = stratum pyramidale; str. rad = stratum radiatum; str. mol = stratum moleculare; str. gran = stratum granulosum. Scale bars A-C= 100 µm; D = 50 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36590090), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of mGluR1 by Immunohistochemistry

Detection of mGluR1 by Immunohistochemistry

mGluR1 alpha expression and quantification of immunoreactivity in the subiculum, entorhinal cortex and superior temporal gyrus in human control and Alzheimer’s disease cases. Photomicrographs of representative regions of the subiculum (A), entorhinal cortex (B), and superior temporal gyrus (C) showing mGluR1 alpha (red) and mGluR1 alpha overlaid with NeuN (green) immunoreactivity for representative Alzheimer’s disease and control cases. In the STG, mGluR1 alpha density shows no statistically significant change in AD (white bars; n = 6) compared to control (black bars; n = 7) cases (Unpaired Mann-Whitney test). The figure shows a significant decrease in mGluR1 alpha expression in the subiculum (*p ≤ 0.05) and entorhinal cortex (*p ≤ 0.05) in AD cases. Data is expressed as mean with individual data points representing single cases. AD = Alzheimer’s disease; ECx = entorhinal cortex: STG = superior temporal gyrus. Scale bars A-C = 100 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36590090), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human mGluR1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human mGluR1a

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR1

Metabotropic glutamate receptor 1 (mGluR1) is a 130 kDa, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. On neurons, mGluR1 is postsynaptic, associates with Gq-like proteins, mobilizes intracellular Ca++, and influences ion channel activity. Mature mGluR1 is 1176 amino acids (aa) in length and contains a 574 aa N-terminal extracellular domain (ECD) (aa 19‑592). The ECD binds glutamate and forms either a covalent homodimer or heterodimer with CaSR. There is one alternative splice form for human mGluR1 that shows a 20 aa substitution for the C-terminal 308 amino acids. Over aa 33‑522, human mGluR1 shares more that 98% aa identity with mouse, rat and canine mGluR1.

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

2911 (Human); 14816 (Mouse); 24414 (Rat)

Gene Symbol

GRM1

UniProt

Q13255

Additional mGluR1 Products

Product Documents for Human mGluR1 Antibody

Certificate of Analysis

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Product Specific Notices for Human mGluR1 Antibody

For research use only

Citations for Human mGluR1 Antibody

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