Human MLK2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human MLK2 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human MLK2 Antibody Summary

Species Reactivity
Detects endogenous human MLK2 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human MLK2
Accession # Q02779
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MLK2 antibody by Western Blot. View Larger

Detection of Human MLK2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia, and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5066) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK2 at approximately 104 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MLK2

MLK2 (mixed lineage kinase 2; also MST and MAP3K10) is a member of the STE Ser/Thr kinase family of enzymes. It is expressed in neurons, lymphoid tissue, and skeletal muscle, where it participates in apoptosis. Human MLK2 is 954 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 23‑76), followed by a kinase domain that likely acts to phosphorylate Ser/Thr (aa 99‑355), two Leu-zipper segments that mediate homodimerization (aa 384‑419) and a C-terminal Ser/Thr/Pro-rich region that undergoes phosphorylation at multiple sites (aa 497‑954). There is one splice variant that shows an in-frame 16 aa substitution for the 16 aa between aa 465‑480.

Long Name
Mixed Lineage Kinase 2
Entrez Gene IDs
4294 (Human); 269881 (Mouse); 308463 (Rat)
Alternate Names
EC 2.7.11; MAP3K10; MEKK10; mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 kinase; MLK2; MLK2MKN28 derived nonreceptor_type serine/threonine kinase; MST; MSTEC; Protein kinase MST

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Citation for Human MLK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. DNA methylation?regulated miR?155?5p depresses sensitivity of esophageal carcinoma cells to radiation and multiple chemotherapeutic drugs via suppression of MAP3K10
    Authors: W Luo, H Zhang, X Liang, R Xia, H Deng, Q Yi, L Lv, L Qian
    Oncol. Rep., 2020;43(5):1692-1704.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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