Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MLK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MLK2
His345-Arg495
Accession # Q02779

Specificity

Detects endogenous human MLK2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human MLK2 Antibody

Detection of Human MLK2 antibody by Western Blot.

Detection of Human MLK2 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia, and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5066) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK2 at approximately 104 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human MLK2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia, and U266 human myeloma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MLK2

MLK2 (mixed lineage kinase 2; also MST and MAP3K10) is a member of the STE Ser/Thr kinase family of enzymes. It is expressed in neurons, lymphoid tissue, and skeletal muscle, where it participates in apoptosis. Human MLK2 is 954 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 23‑76), followed by a kinase domain that likely acts to phosphorylate Ser/Thr (aa 99‑355), two Leu-zipper segments that mediate homodimerization (aa 384‑419) and a C-terminal Ser/Thr/Pro-rich region that undergoes phosphorylation at multiple sites (aa 497‑954). There is one splice variant that shows an in-frame 16 aa substitution for the 16 aa between aa 465‑480.

Long Name

Mixed Lineage Kinase 2

Alternate Names

MAP3K10, MST

Entrez Gene IDs

4294 (Human); 269881 (Mouse); 308463 (Rat)

Gene Symbol

MAP3K10

UniProt

Q02779 (Human)

Additional MLK2 Products

Product Documents for Human MLK2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MLK2 Antibody

For research use only

Citations for Human MLK2 Antibody

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