Key Product Details

Species Reactivity

Human, Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Sheep IgG
View all Arginase 1/ARG1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Arginase 1/ARG1
Accession # P05089

Specificity

Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse Arginase 1/ARG1 Alexa Fluor® 700‑conjugated Antibody

Detection of Arginase 1/ARG1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868N, filled histogram) or isotype control antibody (Catalog # IC016N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins.
Detection of Arginase 1/ARG1 antibody in Hepa 1-6 Mouse Cell Line antibody by Flow Cytometry.

Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry.

Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868N, filled histogram) or isotype control antibody (Catalog # IC016N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins.
Detection of Arginase 1/ARG1 by Flow Cytometry

Detection of Arginase 1/ARG1 by Flow Cytometry

CD45+CECs in COVID-19 patients are more potent. (A) Representative flow cytometry plots of the gating strategy for CD45− and CD45+CECs, and the expression of ROS and arginase I in CD45− versus CD45+CECs. (B) Cumulative data of the intensity of ROS expression measured by mean fluorescence intensity (MFI) in CD45− versus CD45+CECs. (C) Cumulative data of the intensity (MFI) of arginase I expression in CD45− versus CD45+CECs. (D) Representative flow cytometry plots, and (E) cumulative data showing % CD8+ T cells expressing GzmB, or (F) CD107a without stimulation (unstim) or stimulated (stim) with anti-CD3/CD28 antibodies in the absence of CECs (−CECs) or presence (+CECs) without and with l-arginine supplementation (2 mM). (G) Cumulative data showing % CD8+ T cells expressing GzmB, or (H) CD107a without stimulation (Unstim) or stimulated (stim) with anti-CD3/CD28 antibodies in the absence of CECs (−CECs) or presence (+CECs). Each dot represents data from a human subject. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35943266), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Arginase 1/ARG1 by Flow Cytometry

Detection of Arginase 1/ARG1 by Flow Cytometry

CD45+CECs in COVID-19 patients are more potent. (A) Representative flow cytometry plots of the gating strategy for CD45− and CD45+CECs, and the expression of ROS and arginase I in CD45− versus CD45+CECs. (B) Cumulative data of the intensity of ROS expression measured by mean fluorescence intensity (MFI) in CD45− versus CD45+CECs. (C) Cumulative data of the intensity (MFI) of arginase I expression in CD45− versus CD45+CECs. (D) Representative flow cytometry plots, and (E) cumulative data showing % CD8+ T cells expressing GzmB, or (F) CD107a without stimulation (unstim) or stimulated (stim) with anti-CD3/CD28 antibodies in the absence of CECs (−CECs) or presence (+CECs) without and with l-arginine supplementation (2 mM). (G) Cumulative data showing % CD8+ T cells expressing GzmB, or (H) CD107a without stimulation (Unstim) or stimulated (stim) with anti-CD3/CD28 antibodies in the absence of CECs (−CECs) or presence (+CECs). Each dot represents data from a human subject. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35943266), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Arginase 1/ARG1 Alexa Fluor® 700‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: HepG2 Human Cell Line and Hepa 1-6 Mouse Cell Line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Long Name

Liver-Type Arginase

Alternate Names

AI, ARG1, Arginase-1, Liver Arginase, PGIF, Type I Arginase

Entrez Gene IDs

383 (Human); 11846 (Mouse); 29221 (Rat)

Gene Symbol

ARG1

UniProt

P05089

Additional Arginase 1/ARG1 Products

Product Documents for Human/Mouse Arginase 1/ARG1 Alexa Fluor® 700‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Arginase 1/ARG1 Alexa Fluor® 700‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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