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Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody

Catalog #: IC5868F
18 Citations 1 Review Datasheet / COA / SDS
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IC5868F
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry.
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Citations (18)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Arginase 1/ARG1
Met1-Lys322
Accession # P05089
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Arginase 1/ARG1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or isotype control antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Arginase 1/ARG1 antibody in Hepa 1-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Arginase 1/ARG1 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or isotype control antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Long Name
Liver-Type Arginase
Entrez Gene IDs
383 (Human); 11846 (Mouse); 29221 (Rat)
Alternate Names
AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; EC:3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase

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Citations for Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Pneumococcal Extracellular Vesicles Modulate Host Immunity
    Authors: SS Yerneni, S Werner, JH Azambuja, N Ludwig, R Eutsey, SD Aggarwal, PC Lucas, N Bailey, TL Whiteside, PG Campbell, NL Hiller
    MBio, 2021;0(0):e0165721.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Nod1 promotes colorectal carcinogenesis by regulating the immunosuppressive functions of tumor-infiltrating myeloid cells
    Authors: C Maisonneuv, DKL Tsang, EG Foerster, LM Robert, T Mukherjee, D Prescott, I Tattoli, P Lemire, DA Winer, S Winer, CJ Streutker, K Geddes, K Cadwell, RL Ferrero, A Martin, SE Girardin, DJ Philpott
    Cell Reports, 2021;34(4):108677.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Dysregulated Microglial Cell Activation and Proliferation Following Repeated Antigen Stimulation
    Authors: S Prasad, WS Sheng, S Hu, P Chauhan, JR Lokensgard
    Frontiers in Cellular Neuroscience, 2021;15(0):686340.
    Species: Mice
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy
    Authors: A Rodriguez-, RC Lynn, M Poussin, MA Eiva, LC Shaw, RS O'Connor, NG Minutolo, V Casado-Med, G Lopez, T Matsuyama, DJ Powell
    Nature Communications, 2021;12(1):877.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi
    Authors: X Tan, P Banerjee, L Shi, GY Xiao, BL Rodriguez, CL Grzeskowia, X Liu, J Yu, DL Gibbons, WK Russell, CJ Creighton, JM Kurie
    Science Advances, 2021;7(25):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ICC
  6. Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage differentiation in the colon
    Authors: A Nakamura, S Kurihara, D Takahashi, W Ohashi, Y Nakamura, S Kimura, M Onuki, A Kume, Y Sasazawa, Y Furusawa, Y Obata, S Fukuda, S Saiki, M Matsumoto, K Hase
    Nature Communications, 2021;12(1):2105.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Myeloid-derived suppressor cell depletion therapy targets IL-17A-expressing mammary carcinomas
    Authors: B Dawod, J Liu, S Gebremeske, C Yan, A Sappong, B Johnston, DW Hoskin, JS Marshall, J Wang
    Sci Rep, 2020;10(1):13343.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Regulatory T Cells Play a Role in a Subset of Idiopathic Preterm Labor/Birth and Adverse Neonatal Outcomes
    Authors: N Gomez-Lope, M Arenas-Her, R Romero, D Miller, V Garcia-Flo, Y Leng, Y Xu, J Galaz, SS Hassan, CD Hsu, H Tse, C Sanchez-To, B Done, AL Tarca
    Cell Rep, 2020;32(1):107874.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Hippocampal neural stem cells and microglia response to experimental inflammatory bowel disease (IBD)
    Authors: IA Gampieraki, Y Koutmani, M Semitekolo, I Morianos, I Charalampo, G Xanthou, A Gravanis, KP Karalis
    Mol. Psychiatry, 2020;0(0):.
    Species: Mouse
    Sample Types: Tissue
    Applications: Flow Cytometry
  10. Astragalus Polysaccharide RAP Induces Macrophage Phenotype Polarization to M1 via the Notch Signaling Pathway
    Authors: W Wei, ZP Li, ZX Bian, QB Han
    Molecules, 2019;24(10):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Phosphorylated exogenous alpha-synuclein fibrils exacerbate pathology and induce neuronal dysfunction in mice
    Authors: M Karampetso, MT Ardah, M Semitekolo, A Polissidis, M Samiotaki, M Kalomoiri, N Majbour, G Xanthou, OMA El-Agnaf, K Vekrellis
    Sci Rep, 2017;7(1):16533.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. NK Cells Alleviate Lung Inflammation by Negatively Regulating Group 2 Innate Lymphoid Cells
    Authors: J Bi, L Cui, G Yu, X Yang, Y Chen, X Wan
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Modulation of Microglial Cell Fc? Receptor Expression Following Viral Brain Infection
    Authors: P Chauhan, S Hu, WS Sheng, S Prasad, JR Lokensgard
    Sci Rep, 2017;7(0):41889.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Systemic T Cells Immunosuppression of Glioma Stem Cell-Derived Exosomes Is Mediated by Monocytic Myeloid-Derived Suppressor Cells
    Authors: R Domenis, D Cesselli, B Toffoletto, E Bourkoula, F Caponnetto, I Manini, AP Beltrami, T Ius, M Skrap, C Di Loreto, G Gri
    PLoS ONE, 2017;12(1):e0169932.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Targeting Ornithine Decarboxylase by ?-Difluoromethylornithine Inhibits Tumor Growth by Impairing Myeloid-Derived Suppressor Cells.
    Authors: Cong Ye, Zhe Geng, Donye Dominguez, Siqi Chen, Jie Fan, Lei Qin, Alan Long, Yi Zhang, Timothy M Kuzel, Bin Zhang
    Journal of Immunology (Baltimore, Md. : 1950), 2016;0(0):1550-6606.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Increased levels of granulocytic myeloid-derived suppressor cells in peripheral blood and tumour tissue of pancreatic cancer patients.
    Authors: Khaled, Yazan S, Ammori, Basil J, Elkord, Eyad
    J Immunol Res, 2014;2014(0):879897.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  17. Immunosuppressive CD71+ erythroid cells compromise neonatal host defence against infection.
    Authors: Elahi, Shokroll, Ertelt, James M, Kinder, Jeremy M, Jiang, Tony T, Zhang, Xuzhe, Xin, Lijun, Chaturvedi, Vandana, Strong, Beverly, Qualls, Joseph E, Steinbrecher, Kris A, Kalfa, Theodosi, Shaaban, Aimen F, Way, Sing Sin
    Nature, 2013;504(7478):158-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Myeloid-derived suppressor cell measurements in fresh and cryopreserved blood samples.
    J. Immunol. Methods, 2012;381(1):14-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Normal Sheep IgG Fluorescein Control

IC016F
1 Review
1 Image
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Staining Reagents

Flow Cytometry Fixation & Permeabilization Buffer Kit I

FC009
12 Citations
1 Review
Flow

Flow Cytometry Fixation Buffer (1X)

FC004
11 Citations
5 Reviews
Flow

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

FC007
8 Citations
1 Review
Flow

Flow Cytometry Permeabilization/Wash Buffer I (1X)

FC005
16 Citations
3 Reviews
Flow

Reviews for Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody

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Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody
By Patrick Duncker on 08/18/2017
Application: Flow Sample Tested: Monocytes Species: Mouse
Flow Cytometry Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody IC5868F