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Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody

  (7 citations)
(1 Review)     
Datasheet / CoA / SDS
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry.

Intracellular Staining by Flow Cytometry

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Detection of Arginase 1/ARG1 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry.

Intracellular Staining by Flow Cytometry

View Details
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Arginase 1/ARG1
    Met1-Lys322
    Accession # P05089
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry Arginase 1/ARG1/liver Arginase Antibody [Fluorescein]
click image to view larger
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody
(Catalog # IC5868F, filled histogram) or isotype control antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I
(Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry Arginase 1/ARG1/liver Arginase Antibody [Fluorescein]
click image to view larger
Detection of Arginase 1/ARG1 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry. Hepa 1‑6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or isotype control antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

  • Long Name:
    Liver-Type Arginase
  • Entrez Gene IDs:
    383 (Human); 11846 (Mouse); 29221 (Rat)
  • Alternate Names:
    AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
 Applications
 Sample Type
  1. Systemic T Cells Immunosuppression of Glioma Stem Cell-Derived Exosomes Is Mediated by Monocytic Myeloid-Derived Suppressor Cells
    Authors: R Domenis, D Cesselli, B Toffoletto, E Bourkoula, F Caponnetto, I Manini, AP Beltrami, T Ius, M Skrap, C Di Loreto, G Gri
    PLoS ONE, 2017;12(1):e0169932.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Modulation of Microglial Cell Fc? Receptor Expression Following Viral Brain Infection
    Authors: P Chauhan, S Hu, WS Sheng, S Prasad, JR Lokensgard
    Sci Rep, 2017;7(0):41889.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. NK Cells Alleviate Lung Inflammation by Negatively Regulating Group 2 Innate Lymphoid Cells
    Authors: J Bi, L Cui, G Yu, X Yang, Y Chen, X Wan
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Phosphorylated exogenous alpha-synuclein fibrils exacerbate pathology and induce neuronal dysfunction in mice
    Authors: M Karampetso, MT Ardah, M Semitekolo, A Polissidis, M Samiotaki, M Kalomoiri, N Majbour, G Xanthou, OMA El-Agnaf, K Vekrellis
    Sci Rep, 2017;7(1):16533.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Increased levels of granulocytic myeloid-derived suppressor cells in peripheral blood and tumour tissue of pancreatic cancer patients.
    Authors: Khaled, Yazan S, Ammori, Basil J, Elkord, Eyad
    J Immunol Res, 2014;2014(0):879897.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow
  6. Immunosuppressive CD71+ erythroid cells compromise neonatal host defence against infection.
    Authors: Elahi, Shokroll, Ertelt, James M, Kinder, Jeremy M, Jiang, Tony T, Zhang, Xuzhe, Xin, Lijun, Chaturvedi, Vandana, Strong, Beverly, Qualls, Joseph E, Steinbrecher, Kris A, Kalfa, Theodosi, Shaaban, Aimen F, Way, Sing Sin
    Nature, 2013;504(7478):158-62.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Myeloid-derived suppressor cell measurements in fresh and cryopreserved blood samples.
    J. Immunol. Methods, 2012;381(1):14-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Fluorescein Control

 Ctrl IC016F  
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Staining Reagents
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Flow Cytometry Permeabilization/Wash Buffer I (1X)

 Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

 Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

 Flow FC009 4
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

 Flow FC007 2
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody IC5868F Array IC5868F
Enlarge 		Excellent
  Flow Mouse Anonymous 08/18/2017
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Flow Cytometry Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody IC5868F
Flow Cytometry: Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody [IC5868F].

Summary

Application Flow Cytometry
Sample Tested Monocytes
Species Mouse

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