Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody Summary
Met1-Lys322
Accession # P05089
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or isotype control antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Arginase 1/ARG1 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or isotype control antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Arginase 1/ARG1
Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.
Product Datasheets
Citations for Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
18
Citations: Showing 1 - 10
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Pneumococcal Extracellular Vesicles Modulate Host Immunity
Authors: SS Yerneni, S Werner, JH Azambuja, N Ludwig, R Eutsey, SD Aggarwal, PC Lucas, N Bailey, TL Whiteside, PG Campbell, NL Hiller
MBio, 2021;0(0):e0165721.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Nod1 promotes colorectal carcinogenesis by regulating the immunosuppressive functions of tumor-infiltrating myeloid cells
Authors: C Maisonneuv, DKL Tsang, EG Foerster, LM Robert, T Mukherjee, D Prescott, I Tattoli, P Lemire, DA Winer, S Winer, CJ Streutker, K Geddes, K Cadwell, RL Ferrero, A Martin, SE Girardin, DJ Philpott
Cell Reports, 2021;34(4):108677.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dysregulated Microglial Cell Activation and Proliferation Following Repeated Antigen Stimulation
Authors: S Prasad, WS Sheng, S Hu, P Chauhan, JR Lokensgard
Frontiers in Cellular Neuroscience, 2021;15(0):686340.
Species: Mice
Sample Types: Whole Cells
Applications: Flow Cytometry -
CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy
Authors: A Rodriguez-, RC Lynn, M Poussin, MA Eiva, LC Shaw, RS O'Connor, NG Minutolo, V Casado-Med, G Lopez, T Matsuyama, DJ Powell
Nature Communications, 2021;12(1):877.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi
Authors: X Tan, P Banerjee, L Shi, GY Xiao, BL Rodriguez, CL Grzeskowia, X Liu, J Yu, DL Gibbons, WK Russell, CJ Creighton, JM Kurie
Science Advances, 2021;7(25):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ICC -
Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage differentiation in the colon
Authors: A Nakamura, S Kurihara, D Takahashi, W Ohashi, Y Nakamura, S Kimura, M Onuki, A Kume, Y Sasazawa, Y Furusawa, Y Obata, S Fukuda, S Saiki, M Matsumoto, K Hase
Nature Communications, 2021;12(1):2105.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Myeloid-derived suppressor cell depletion therapy targets IL-17A-expressing mammary carcinomas
Authors: B Dawod, J Liu, S Gebremeske, C Yan, A Sappong, B Johnston, DW Hoskin, JS Marshall, J Wang
Sci Rep, 2020;10(1):13343.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulatory T Cells Play a Role in a Subset of Idiopathic Preterm Labor/Birth and Adverse Neonatal Outcomes
Authors: N Gomez-Lope, M Arenas-Her, R Romero, D Miller, V Garcia-Flo, Y Leng, Y Xu, J Galaz, SS Hassan, CD Hsu, H Tse, C Sanchez-To, B Done, AL Tarca
Cell Rep, 2020;32(1):107874.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hippocampal neural stem cells and microglia response to experimental inflammatory bowel disease (IBD)
Authors: IA Gampieraki, Y Koutmani, M Semitekolo, I Morianos, I Charalampo, G Xanthou, A Gravanis, KP Karalis
Mol. Psychiatry, 2020;0(0):.
Species: Mouse
Sample Types: Tissue
Applications: Flow Cytometry -
Astragalus Polysaccharide RAP Induces Macrophage Phenotype Polarization to M1 via the Notch Signaling Pathway
Authors: W Wei, ZP Li, ZX Bian, QB Han
Molecules, 2019;24(10):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Phosphorylated exogenous alpha-synuclein fibrils exacerbate pathology and induce neuronal dysfunction in mice
Authors: M Karampetso, MT Ardah, M Semitekolo, A Polissidis, M Samiotaki, M Kalomoiri, N Majbour, G Xanthou, OMA El-Agnaf, K Vekrellis
Sci Rep, 2017;7(1):16533.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
NK Cells Alleviate Lung Inflammation by Negatively Regulating Group 2 Innate Lymphoid Cells
Authors: J Bi, L Cui, G Yu, X Yang, Y Chen, X Wan
J. Immunol, 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Modulation of Microglial Cell Fc? Receptor Expression Following Viral Brain Infection
Authors: P Chauhan, S Hu, WS Sheng, S Prasad, JR Lokensgard
Sci Rep, 2017;7(0):41889.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Systemic T Cells Immunosuppression of Glioma Stem Cell-Derived Exosomes Is Mediated by Monocytic Myeloid-Derived Suppressor Cells
Authors: R Domenis, D Cesselli, B Toffoletto, E Bourkoula, F Caponnetto, I Manini, AP Beltrami, T Ius, M Skrap, C Di Loreto, G Gri
PLoS ONE, 2017;12(1):e0169932.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting Ornithine Decarboxylase by ?-Difluoromethylornithine Inhibits Tumor Growth by Impairing Myeloid-Derived Suppressor Cells.
Authors: Cong Ye, Zhe Geng, Donye Dominguez, Siqi Chen, Jie Fan, Lei Qin, Alan Long, Yi Zhang, Timothy M Kuzel, Bin Zhang
Journal of Immunology (Baltimore, Md. : 1950), 2016;0(0):1550-6606.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increased levels of granulocytic myeloid-derived suppressor cells in peripheral blood and tumour tissue of pancreatic cancer patients.
Authors: Khaled, Yazan S, Ammori, Basil J, Elkord, Eyad
J Immunol Res, 2014;2014(0):879897.
Species: Human
Sample Types: Whole Blood
Applications: Flow Cytometry -
Immunosuppressive CD71+ erythroid cells compromise neonatal host defence against infection.
Authors: Elahi, Shokroll, Ertelt, James M, Kinder, Jeremy M, Jiang, Tony T, Zhang, Xuzhe, Xin, Lijun, Chaturvedi, Vandana, Strong, Beverly, Qualls, Joseph E, Steinbrecher, Kris A, Kalfa, Theodosi, Shaaban, Aimen F, Way, Sing Sin
Nature, 2013;504(7478):158-62.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Myeloid-derived suppressor cell measurements in fresh and cryopreserved blood samples.
J. Immunol. Methods, 2012;381(1):14-22.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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