Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody
Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody Summary
Met1-Lys322
Accession # P05089
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Arginase 1/ARG1
Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.
Product Datasheets
Citations for Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Helminth resistance is mediated by differential activation of recruited monocyte-derived alveolar macrophages and arginine depletion
Authors: F Chen, DW El-Naccach, JJ Ponessa, A Lemenze, V Espinosa, W Wu, K Lothstein, L Jin, O Antao, JS Weinstein, P Damani-Yok, K Khanna, PJ Murray, A Rivera, MC Siracusa, WC Gause
Cell Reports, 2022;38(2):110215.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Topical arginase inhibition decreases growth of cutaneous squamous cell carcinoma
Authors: A Mittal, M Wang, A Vidyarthi, D Yanez, G Pizzurro, D Thakral, E Tracy, OR Colegio
Scientific Reports, 2021;11(1):10731.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke
Authors: M Hu, Y Lin, X Men, S Wang, X Sun, Q Zhu, D Lu, S Liu, B Zhang, W Cai, Z Lu
Journal of Neuroinflammation, 2021;18(1):90.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Midkine rewires the melanoma microenvironment toward a tolerogenic and immune-resistant state
Authors: D Cerezo-Wal, M Contreras-, K Troulé, X Catena, C Mucientes, TG Calvo, E Cañón, C Tejedo, PC Pennacchi, S Hogan, P Kölblinger, H Tejero, AX Chen, N Ibarz, O Graña-Cast, L Martinez, J Muñoz, P Ortiz-Rome, JL Rodriguez-, G Gómez-Lópe, F Al-Shahrou, R Rabadán, MP Levesque, D Olmeda, MS Soengas
Nat Med, 2020;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
DEK Expression in Breast Cancer Cells Leads to the Alternative Activation of Tumor Associated Macrophages
Authors: NA Pease, MS Shephard, M Sertorio, SE Waltz, LMP Vinnedge
Cancers (Basel), 2020;12(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR
Authors: W Nowak, LN Grendas, LM Sanmarco, IG Estecho, ÁR Arena, N Eberhardt, DE Rodante, MP Aoki, FM Daray, EA Carrera Si, AE Errasti
EBioMedicine, 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Taenia crassiceps-Excreted/Secreted Products Induce a Defined MicroRNA Profile that Modulates Inflammatory Properties of Macrophages
Authors: D Martínez-S, JD Ruíz-Rosad, C Terrazas, BE Callejas, AR Satoskar, S Partida-Sá, LI Terrazas
J Immunol Res, 2019;2019(0):2946713.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
U3-1402 sensitizes HER3-expressing tumors to PD-1 blockade by immune activation
Authors: K Haratani, K Yonesaka, S Takamura, O Maenishi, R Kato, N Takegawa, H Kawakami, K Tanaka, H Hayashi, M Takeda, N Maeda, T Kagari, K Hirotani, J Tsurutani, K Nishio, K Doi, M Miyazawa, K Nakagawa
J. Clin. Invest., 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions
Authors: PT Mantani, P Dunér, I Ljungcrant, J Nilsson, H Björkbacka, GN Fredrikson
BMC Immunol., 2019;20(1):47.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice
Authors: W Cai, X Dai, J Chen, J Zhao, M Xu, L Zhang, B Yang, W Zhang, M Rocha, T Nakao, J Kofler, Y Shi, RA Stetler, X Hu, J Chen
JCI Insight, 2019;4(20):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
All trans-retinoic acid protects against acute ischemic stroke by modulating neutrophil functions through STAT1 signaling
Authors: W Cai, J Wang, M Hu, X Chen, Z Lu, JA Bellanti, SG Zheng
J Neuroinflammation, 2019;16(1):175.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages
Authors: EA Ayaub, K Tandon, M Padwal, J Imani, H Patel, A Dubey, O Mekhael, C Upagupta, A Ayoub, A Dvorkin-Gh, J Murphy, PS Kolb, S Lhotak, JG Dickhout, RC Austin, MR Kolb, CD Richards, K Ask
Immunol. Cell Biol., 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury
Authors: DD Pearse, J Bastidas, SS Izabel, M Ghosh
Int J Mol Sci, 2018;19(9):.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
CCR2-dependent monocytes/macrophages exacerbate acute brain injury but promote functional recovery after ischemic stroke in mice
Authors: W Fang, X Zhai, D Han, X Xiong, T Wang, X Zeng, S He, R Liu, M Miyata, B Xu, H Zhao
Theranostics, 2018;8(13):3530-3543.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis
Authors: EA Ayaub, A Dubey, J Imani, F Botelho, MRJ Kolb, CD Richards, K Ask
Sci Rep, 2017;7(1):13281.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer
Authors: RY Huang, A Francois, AR McGray, A Miliotto, K Odunsi
Oncoimmunology, 2017;6(1):e1249561.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE.
Authors: Moreno M, Bannerman P, Ma J, Guo F, Miers L, Soulika A, Pleasure D
J Neurosci, 2014;34(24):8175-85.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
Authors: Mills Ko E, Ma J, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika A, Pleasure D
J Neuroinflammation, 2014;11(0):105.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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