Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5868A
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (18)
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Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Arginase 1/ARG1
Met1-Lys322
Accession # P05089
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Arginase 1/ARG1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Arginase 1/ARG1 antibody in Hepa 1-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Long Name
Liver-Type Arginase
Entrez Gene IDs
383 (Human); 11846 (Mouse); 29221 (Rat)
Alternate Names
AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; EC:3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase

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Citations for Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Helminth resistance is mediated by differential activation of recruited monocyte-derived alveolar macrophages and arginine depletion
    Authors: F Chen, DW El-Naccach, JJ Ponessa, A Lemenze, V Espinosa, W Wu, K Lothstein, L Jin, O Antao, JS Weinstein, P Damani-Yok, K Khanna, PJ Murray, A Rivera, MC Siracusa, WC Gause
    Cell Reports, 2022;38(2):110215.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  2. Topical arginase inhibition decreases growth of cutaneous squamous cell carcinoma
    Authors: A Mittal, M Wang, A Vidyarthi, D Yanez, G Pizzurro, D Thakral, E Tracy, OR Colegio
    Scientific Reports, 2021;11(1):10731.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke
    Authors: M Hu, Y Lin, X Men, S Wang, X Sun, Q Zhu, D Lu, S Liu, B Zhang, W Cai, Z Lu
    Journal of Neuroinflammation, 2021;18(1):90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Midkine rewires the melanoma microenvironment toward a tolerogenic and immune-resistant state
    Authors: D Cerezo-Wal, M Contreras-, K Troulé, X Catena, C Mucientes, TG Calvo, E Cañón, C Tejedo, PC Pennacchi, S Hogan, P Kölblinger, H Tejero, AX Chen, N Ibarz, O Graña-Cast, L Martinez, J Muñoz, P Ortiz-Rome, JL Rodriguez-, G Gómez-Lópe, F Al-Shahrou, R Rabadán, MP Levesque, D Olmeda, MS Soengas
    Nat Med, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. DEK Expression in Breast Cancer Cells Leads to the Alternative Activation of Tumor Associated Macrophages
    Authors: NA Pease, MS Shephard, M Sertorio, SE Waltz, LMP Vinnedge
    Cancers (Basel), 2020;12(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR
    Authors: W Nowak, LN Grendas, LM Sanmarco, IG Estecho, ÁR Arena, N Eberhardt, DE Rodante, MP Aoki, FM Daray, EA Carrera Si, AE Errasti
    EBioMedicine, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Taenia crassiceps-Excreted/Secreted Products Induce a Defined MicroRNA Profile that Modulates Inflammatory Properties of Macrophages
    Authors: D Martínez-S, JD Ruíz-Rosad, C Terrazas, BE Callejas, AR Satoskar, S Partida-Sá, LI Terrazas
    J Immunol Res, 2019;2019(0):2946713.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. U3-1402 sensitizes HER3-expressing tumors to PD-1 blockade by immune activation
    Authors: K Haratani, K Yonesaka, S Takamura, O Maenishi, R Kato, N Takegawa, H Kawakami, K Tanaka, H Hayashi, M Takeda, N Maeda, T Kagari, K Hirotani, J Tsurutani, K Nishio, K Doi, M Miyazawa, K Nakagawa
    J. Clin. Invest., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions
    Authors: PT Mantani, P Dunér, I Ljungcrant, J Nilsson, H Björkbacka, GN Fredrikson
    BMC Immunol., 2019;20(1):47.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice
    Authors: W Cai, X Dai, J Chen, J Zhao, M Xu, L Zhang, B Yang, W Zhang, M Rocha, T Nakao, J Kofler, Y Shi, RA Stetler, X Hu, J Chen
    JCI Insight, 2019;4(20):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. All trans-retinoic acid protects against acute ischemic stroke by modulating neutrophil functions through STAT1 signaling
    Authors: W Cai, J Wang, M Hu, X Chen, Z Lu, JA Bellanti, SG Zheng
    J Neuroinflammation, 2019;16(1):175.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages
    Authors: EA Ayaub, K Tandon, M Padwal, J Imani, H Patel, A Dubey, O Mekhael, C Upagupta, A Ayoub, A Dvorkin-Gh, J Murphy, PS Kolb, S Lhotak, JG Dickhout, RC Austin, MR Kolb, CD Richards, K Ask
    Immunol. Cell Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury
    Authors: DD Pearse, J Bastidas, SS Izabel, M Ghosh
    Int J Mol Sci, 2018;19(9):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. CCR2-dependent monocytes/macrophages exacerbate acute brain injury but promote functional recovery after ischemic stroke in mice
    Authors: W Fang, X Zhai, D Han, X Xiong, T Wang, X Zeng, S He, R Liu, M Miyata, B Xu, H Zhao
    Theranostics, 2018;8(13):3530-3543.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis
    Authors: EA Ayaub, A Dubey, J Imani, F Botelho, MRJ Kolb, CD Richards, K Ask
    Sci Rep, 2017;7(1):13281.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer
    Authors: RY Huang, A Francois, AR McGray, A Miliotto, K Odunsi
    Oncoimmunology, 2017;6(1):e1249561.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE.
    Authors: Moreno M, Bannerman P, Ma J, Guo F, Miers L, Soulika A, Pleasure D
    J Neurosci, 2014;34(24):8175-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
    Authors: Mills Ko E, Ma J, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika A, Pleasure D
    J Neuroinflammation, 2014;11(0):105.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody
By Adam Guess on 09/07/2016
Application: Flow Sample Tested: Mouse splenocytes Species: Mouse