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Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody

  (4 citations)
(1 Review)     
Datasheet / CoA / SDS
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry.

Intracellular Staining by Flow Cytometry

View Details
Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry.

Intracellular Staining by Flow Cytometry

View Details
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Arginase 1/ARG1
    Met1-Lys322
    Accession # P05089
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry Arginase 1/ARG1/liver Arginase Antibody [Allophycocyanin]
click image to view larger
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry Arginase 1/ARG1/liver Arginase Antibody [Allophycocyanin]
click image to view larger
Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

  • Long Name:
    Liver-Type Arginase
  • Entrez Gene IDs:
    383 (Human); 11846 (Mouse); 29221 (Rat)
  • Alternate Names:
    AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
 Applications
 Sample Type
  1. Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis
    Authors: EA Ayaub, A Dubey, J Imani, F Botelho, MRJ Kolb, CD Richards, K Ask
    Sci Rep, 2017;7(1):13281.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer
    Authors: RY Huang, A Francois, AR McGray, A Miliotto, K Odunsi
    Oncoimmunology, 2017;6(1):e1249561.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
    Authors: Mills Ko E, Ma J, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika A, Pleasure D
    J Neuroinflammation, 2014;11(0):105.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE.
    Authors: Moreno M, Bannerman P, Ma J, Guo F, Miers L, Soulika A, Pleasure D
    J Neurosci, 2014;34(24):8175-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG APC-conjugated Antibody

 Ctrl IC016A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

 Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

 Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

 Flow FC009 4
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

 Flow FC007 2
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Average Rating: 4 (Based on 1 review)

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Read what people are saying who have used Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody.

We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody IC5868A Array IC5868A
Enlarge 		Very Good
  Flow Mouse Anonymous 09/07/2016
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Image Details
Flow Cytometry Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody IC5868A
Flow Cytometry: Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody [IC5868A].

Summary

Application Flow Cytometry
Sample Tested Mouse splenocytes
Species Mouse

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