Human/Mouse Arginase 1/ARG1 PE-conjugated Antibody

(7 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Arginase 1/ARG1
    Met1-Lys322
    Accession # P05089
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

  • Long Name:
    Liver-Type Arginase
  • Entrez Gene IDs:
    383 (Human); 11846 (Mouse); 29221 (Rat)
  • Alternate Names:
    AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Infection-adapted emergency hematopoiesis promotes visceral leishmaniasis
    Authors: BM Abidin, A Hammami, S Stäger, KM Heinonen
    PLoS Pathog., 2017;13(8):e1006422.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Therapeutic benefits of phosphodiesterase 4B inhibition after traumatic brain injury
    Authors: NM Wilson, ME Gurney, WD Dietrich, CM Atkins
    PLoS ONE, 2017;12(5):e0178013.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. IL-4 upregulates cyclooxygenase-1 expression in macrophages
    Authors: AE Shay, BT Diwakar, BJ Guan, V Narayan, JF Urban, KS Prabhu
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Obesity triggers enhanced MDSC accumulation in murine renal tumors via elevated local production of CCL2.
    Authors: Hale, Malika, Itani, Farah, Buchta, Claire M, Wald, Gal, Bing, Megan, Norian, Lyse A
    PLoS ONE, 2015;10(3):e0118784.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow
  5. IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.
    Authors: Baek J, Zeng R, Weinmann-Menke J, Valerius M, Wada Y, Ajay A, Colonna M, Kelley V
    J Clin Invest, 2015;125(8):3198-214.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Protective Role of STAT6 in Basophil-Dependent Prurigo-like Allergic Skin Inflammation.
    Authors: Hashimoto T, Satoh T, Yokozeki H
    J Immunol, 2015;194(10):4631-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using myeloid-derived suppressor cells as a readout of therapeutic efficacy.
    Authors: Liechtenstein T, Perez-Janices N, Blanco-Luquin I, Goyvaerts C, Schwarze J, Dufait I, Lanna A, Ridder M, Guerrero-Setas D, Breckpot K, Escors D
    Oncoimmunology, 2014;3(7):e945378.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG PE-conjugated Antibody

Ctrl IC016P  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Summary

ApplicationFlow Cytometry
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse

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