Human/Mouse Arginase 1/ARG1 PE-conjugated Antibody
Human/Mouse Arginase 1/ARG1 PE-conjugated Antibody Summary
Met1-Lys322
Accession # P05089
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Arginase 1/ARG1
Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.
Product Datasheets
Citations for Human/Mouse Arginase 1/ARG1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Helminth resistance is mediated by differential activation of recruited monocyte-derived alveolar macrophages and arginine depletion
Authors: F Chen, DW El-Naccach, JJ Ponessa, A Lemenze, V Espinosa, W Wu, K Lothstein, L Jin, O Antao, JS Weinstein, P Damani-Yok, K Khanna, PJ Murray, A Rivera, MC Siracusa, WC Gause
Cell Reports, 2022;38(2):110215.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
VEGFR2 activity on myeloid cells mediates immune suppression in the tumor microenvironment
Authors: Y Zhang, H Huang, M Coleman, A Ziemys, P Gopal, SM Kazmi, RA Brekken
JCI Insight, 2021;6(23):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CCR2 Signaling Promotes Brain Infiltration of Inflammatory Monocytes and Contributes to Neuropathology during Cryptococcal Meningoencephalitis
Authors: J Xu, A Ganguly, J Zhao, M Ivey, R Lopez, JJ Osterholze, CS Cho, MA Olszewski
MBio, 2021;0(0):e0107621.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Ubiquitous Overexpression of Chromatin Remodeling Factor SRG3 Exacerbates Atopic Dermatitis in NC/Nga Mice by Enhancing Th2 Immune Responses
Authors: SW Lee, HJ Park, J Jeon, YH Park, TC Kim, SH Jeon, RH Seong, L Van Kaer, S Hong
International Journal of Molecular Sciences, 2021;22(4):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-13 Ameliorates Neuroinflammation and Promotes Functional Recovery after Traumatic Brain Injury
Authors: W Miao, Y Zhao, Y Huang, D Chen, C Luo, W Su, Y Gao
J. Immunol., 2020;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Optimized protocol for the preparation of single cells from cutaneous wounds for flow cytometric cell sorting and analysis of macrophages
Authors: S Shakya, K Asosingh, JA Mack, EV Maytin
MethodsX, 2020;7(0):101027.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice
Authors: W Cai, X Dai, J Chen, J Zhao, M Xu, L Zhang, B Yang, W Zhang, M Rocha, T Nakao, J Kofler, Y Shi, RA Stetler, X Hu, J Chen
JCI Insight, 2019;4(20):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice
Authors: A Carestia, HA Mena, CM Olexen, JM Ortiz Wilc, S Negrotto, AE Errasti, RM Gómez, CN Jenne, EA Carrera Si, M Schattner
Cell Rep, 2019;28(4):896-908.e5.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sitravatinib potentiates immune checkpoint blockade in refractory cancer models
Authors: W Du, H Huang, N Sorrelle, RA Brekken
JCI Insight, 2018;3(21):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Concerted IL-25R and IL-4R? signaling drive innate type 2 effector immunity for optimal helminth expulsion
Authors: KA Smith, S Löser, F Varyani, Y Harcus, HJ McSorley, AN McKenzie, RM Maizels
Elife, 2018;7(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Temporal changes in macrophage phenotype after peripheral nerve injury
Authors: JE Tomlinson, E Žygelyt?, JK Grenier, MG Edwards, J Cheetham
J Neuroinflammation, 2018;15(1):185.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype
Authors: D Chen, J Xie, R Fiskesund, W Dong, X Liang, J Lv, X Jin, J Liu, S Mo, T Zhang, F Cheng, Y Zhou, H Zhang, K Tang, J Ma, Y Liu, B Huang
Nat Commun, 2018;9(1):873.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dysferlinopathy Promotes an Intramuscle Expansion of Macrophages with a Cyto-Destructive Phenotype
Authors: JH Baek, GM Many, FJ Evesson, VR Kelley
Am. J. Pathol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-4 upregulates cyclooxygenase-1 expression in macrophages
Authors: AE Shay, BT Diwakar, BJ Guan, V Narayan, JF Urban, KS Prabhu
J. Biol. Chem., 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Therapeutic benefits of phosphodiesterase 4B inhibition after traumatic brain injury
Authors: NM Wilson, ME Gurney, WD Dietrich, CM Atkins
PLoS ONE, 2017;12(5):e0178013.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Infection-adapted emergency hematopoiesis promotes visceral leishmaniasis
Authors: BM Abidin, A Hammami, S Stäger, KM Heinonen
PLoS Pathog., 2017;13(8):e1006422.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.
Authors: Baek J, Zeng R, Weinmann-Menke J, Valerius M, Wada Y, Ajay A, Colonna M, Kelley V
J Clin Invest, 2015;125(8):3198-214.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Obesity triggers enhanced MDSC accumulation in murine renal tumors via elevated local production of CCL2.
Authors: Hale, Malika, Itani, Farah, Buchta, Claire M, Wald, Gal, Bing, Megan, Norian, Lyse A
PLoS ONE, 2015;10(3):e0118784.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Protective Role of STAT6 in Basophil-Dependent Prurigo-like Allergic Skin Inflammation.
Authors: Hashimoto T, Satoh T, Yokozeki H
J Immunol, 2015;194(10):4631-40.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using myeloid-derived suppressor cells as a readout of therapeutic efficacy.
Authors: Liechtenstein T, Perez-Janices N, Blanco-Luquin I, Goyvaerts C, Schwarze J, Dufait I, Lanna A, Ridder M, Guerrero-Setas D, Breckpot K, Escors D
Oncoimmunology, 2014;3(7):e945378.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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