Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.
Human/Mouse Arginase 1/ARG1 PE‑conjugated Antibody
R&D Systems | Catalog # IC5868P
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Arginase 1/ARG1
Met1-Lys322
Accession # P05089
Met1-Lys322
Accession # P05089
Specificity
Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse Arginase 1/ARG1 PE‑conjugated Antibody
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry.
Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse Arginase 1/ARG1 PE‑conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line and Hepa 1-6 mouse hepatoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Sample: HepG2 human hepatocellular carcinoma cell line and Hepa 1-6 mouse hepatoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Reviewed Applications
Read 2 reviews rated 4 using IC5868P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Arginase 1/ARG1
Long Name
Liver-Type Arginase
Alternate Names
AI, ARG1, Arginase-1, Liver Arginase, PGIF, Type I Arginase
Gene Symbol
ARG1
UniProt
Additional Arginase 1/ARG1 Products
Product Documents for Human/Mouse Arginase 1/ARG1 PE‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse Arginase 1/ARG1 PE‑conjugated Antibody
For research use only
Related Research Areas
Citations for Human/Mouse Arginase 1/ARG1 PE‑conjugated Antibody
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Customer Reviews for Human/Mouse Arginase 1/ARG1 PE‑conjugated Antibody (2)
4 out of 5
2 Customer Ratings
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Application: Flow CytometrySample Tested: brain and spinal cordSpecies: MouseVerified Customer | Posted 02/21/2019CNS cells of EAE mice stained for viable cells, Arg1, CD11b. CD11b+, Arg1 non-stained cells (up) and CD11b+,Arg1-stained cells (down).Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for CD11b, CD19, Arg1, iNOS, IL-6, IL-10, IL-35, TNF and with a marker for dead cells to exclude them. Viable, CD11b+ cells were gated and Arg1 staining is showed in control cells, non-stained for Arg1 (upper plot), and in Arg1-stained cells (lower plot).
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Application: Flow CytometrySample Tested: RAW 264.7 mouse monocyte/macrophage cell lineSpecies: MouseVerified Customer | Posted 05/24/2017
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Protocols
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