Human/Mouse/Rat Arginase 1/ARG1 Antibody

(4 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Arginase 1/ARG1
    Met1-Lys322
    Accession # P05089
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Rat Arginase 1/ARG1 by Western Blot. Western blot shows lysates of human liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human, Mouse, and Rat Arginase 1/ARG1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

  • Long Name:
    Liver-Type Arginase
  • Entrez Gene IDs:
    383 (Human); 11846 (Mouse); 29221 (Rat)
  • Alternate Names:
    AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population
    Authors: DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh
    Nat Commun, 2017;8(0):14919.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Candida albicans Chitin Increases Arginase-1 Activity in Human Macrophages, with an Impact on Macrophage Antimicrobial Functions
    Authors: J Wagener, DM MacCallum, GD Brown, NA Gow
    MBio, 2017;8(1):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Arginase1 Deficiency in Monocytes/Macrophages Upregulates Inducible Nitric Oxide Synthase To Promote Cutaneous Contact Hypersensitivity
    Authors: J Suwanpradi, M Shih, L Pontius, B Yang, A Birukova, E Guttman-Ya, DL Corcoran, LG Que, RM Tighe, AS MacLeod
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. DAMP signaling is a key pathway inducing immune modulation after brain injury.
    Authors: Liesz A, Dalpke A, Mracsko E, Antoine D, Roth S, Zhou W, Yang H, Na S, Akhisaroglu M, Fleming T, Eigenbrod T, Nawroth P, Tracey K, Veltkamp R
    J Neurosci, 2015;35(2):583-98.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human/Mouse/Rat Arginase 1/ARG1 Antibody AF5868
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 Flow Mouse Anonymous 08/24/2017
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Flow Cytometry Human/Mouse/Rat Arginase 1/ARG1 Antibody AF5868
Flow Cytometry: Human/Mouse/Rat Arginase 1/ARG1 Antibody [AF5868]

Summary

ApplicationFlow Cytometry
Sample TestedMature myeloid dendritic cells
SpeciesMouse

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