Human/Mouse/Rat Arginase 1/ARG1 Antibody

Catalog # Availability Size / Price Qty
AF5868
AF5868-SP
Detection of Human and Rat Arginase 1/ARG1 by Western Blot.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat Arginase 1/ARG1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Arginase 1/ARG1
Met1-Lys322
Accession # P05089
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunoprecipitation
25 µg/mL
Cell lysates spiked with Recombinant Human Arginase 1/ARG1 (Catalog # 5868-AR), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat Arginase 1/ARG1 antibody by Western Blot. View Larger

Detection of Human and Rat Arginase 1/ARG1 by Western Blot. Western blot shows lysates of human liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Human, Mouse, and Rat Arginase 1/ARG1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human, Mouse, and Rat Arginase 1/ARG1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Long Name
Liver-Type Arginase
Entrez Gene IDs
383 (Human); 11846 (Mouse); 29221 (Rat)
Alternate Names
AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; EC:3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase

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Citations for Human/Mouse/Rat Arginase 1/ARG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Cell-intrinsic Wnt4 ligand regulates mitochondrial oxidative phosphorylation in macrophages
    Authors: M Tlili, H Acevedo, A Descoteaux, M Germain, KM Heinonen
    The Journal of Biological Chemistry, 2022;0(0):102193.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. PD-L1 engagement on T cells promotes self-tolerance and suppression of neighboring macrophages and effector T cells in cancer
    Authors: B Diskin, S Adam, MF Cassini, G Sanchez, M Liria, B Aykut, C Buttar, E Li, B Sundberg, RD Salas, R Chen, J Wang, M Kim, MS Farooq, S Nguy, C Fedele, KH Tang, T Chen, W Wang, M Hundeyin, JAK Rossi, E Kurz, MIU Haq, J Karlen, E Kruger, Z Sekendiz, D Wu, SAA Shadaloey, G Baptiste, G Werba, S Selvaraj, C Loomis, KK Wong, J Leinwand, G Miller
    Nat. Immunol., 2020;21(4):442-454.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery
    Authors: W Ren, J Hou, C Yang, H Wang, S Wu, Y Wu, X Zhao, C Lu
    J. Exp. Clin. Cancer Res., 2019;38(1):62.
    Species: Xenograft
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease
    Authors: H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering
    Acta Neuropathol Commun, 2019;7(1):13.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Magnetic Field Changes Macrophage Phenotype
    Authors: J Wosik, W Chen, K Qin, RM Ghobrial, JZ Kubiak, M Kloc
    Biophys. J., 2018;114(8):2001-2013.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population
    Authors: DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh
    Nat Commun, 2017;8(0):14919.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Arginase1 Deficiency in Monocytes/Macrophages Upregulates Inducible Nitric Oxide Synthase To Promote Cutaneous Contact Hypersensitivity
    Authors: J Suwanpradi, M Shih, L Pontius, B Yang, A Birukova, E Guttman-Ya, DL Corcoran, LG Que, RM Tighe, AS MacLeod
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Candida albicans Chitin Increases Arginase-1 Activity in Human Macrophages, with an Impact on Macrophage Antimicrobial Functions
    Authors: J Wagener, DM MacCallum, GD Brown, NA Gow
    MBio, 2017;8(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Assessment of Preclinical Liver and Skeletal Muscle Biomarkers Following Clofibrate Administration in Wistar Rats
    Authors: P Maliver, M Festag, M Bennecke, F Christen, B Bánfai, B Lenz, M Winter
    Toxicol Pathol, 2017;0(0):1926233177072.
    Species: Rat
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  10. DAMP signaling is a key pathway inducing immune modulation after brain injury.
    Authors: Liesz A, Dalpke A, Mracsko E, Antoine D, Roth S, Zhou W, Yang H, Na S, Akhisaroglu M, Fleming T, Eigenbrod T, Nawroth P, Tracey K, Veltkamp R
    J Neurosci, 2015;35(2):583-98.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Arginase 1/ARG1 Antibody

Average Rating: 3.5 (Based on 2 Reviews)

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Human/Mouse/Rat Arginase 1/ARG1 Antibody
By Anonymous on 02/17/2020
Application: WB Sample Tested: endothelial cells Species: Mouse

Human/Mouse/Rat Arginase 1/ARG1 Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Mature myeloid dendritic cells Species: Mouse