Human/Mouse ATG12 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB6807U Datasheet / COA / SDS

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FAB6807U-100UG

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All ATG12 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse ATG12 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human ATG12 in direct ELISAs, and human and mouse ATG12 in Western blots.

Source

Monoclonal Mouse IgG2b Clone # 628902

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human ATG12
Asp48-Ala187
Accession # O94817

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ATG12

ATG12 is a 15 kDa cytosolic protein with a C-terminal ubiquitin-like domain. The conjugation of ATG12 to ATG5 is required for the autophagosome pathway of bulk degradation. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. The ATG5:ATG12 conjugate also inhibits type I interferon-induced antiviral responses and promotes the lipidation of ATG8. Alternate splicing of human ATG12 generates an isoform that lacks the ubiquitin-like domain. Human ATG12 shares 89% and 85% aa sequence identitiy with mouse and rat ATG12, respectively.

Long Name

ATG12 Autophagy Related 12 Homolog

Entrez Gene IDs

9140 (Human); 67526 (Mouse); 361321 (Rat)

Alternate Names

Apg12 (autophagy 12, S. cerevisiae)-like; APG12 autophagy 12-like (S. cerevisiae); APG12; APG12HAPG12; APG12-like; ATG12 autophagy related 12 homolog (S. cerevisiae); ATG12; Autophagy-related protein 12; FBR93; HAPG12; ubiquitin-like protein ATG12; yeast) homolog