Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 628902
View all ATG12 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ATG12
Asp48-Ala187
Accession # O94817

Specificity

Detects human ATG12 in direct ELISAs, and human and mouse ATG12 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse ATG12 Antibody

Detection of Human and Mouse ATG12 antibody by Western Blot.

Detection of Human and Mouse ATG12 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane (left) was probed with 0.1 µg/mL of Mouse Anti-Human ATG12 Monoclonal Antibody (Catalog # MAB6807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane (right) was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the ATG5:ATG12 heterodimer at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
ATG12 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC).

ATG12 in RAW 264.7 Mouse Cell Line.

ATG12 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line untreated (lower panel) or stimulated with LPS (upper panel) using Mouse Anti-Human/Mouse ATG12 Monoclonal Antibody (Catalog # MAB6807) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human/Mouse ATG12 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HCT‑116 human colorectal carcinoma cell line, and C2C12 mouse myoblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB6807 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ATG12

ATG12 is a 15 kDa cytosolic protein with a C-terminal ubiquitin-like domain. The conjugation of ATG12 to ATG5 is required for the autophagosome pathway of bulk degradation. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. The ATG5:ATG12 conjugate also inhibits type I interferon-induced antiviral responses and promotes the lipidation of ATG8. Alternate splicing of human ATG12 generates an isoform that lacks the ubiquitin-like domain. Human ATG12 shares 89% and 85% aa sequence identitiy with mouse and rat ATG12, respectively.

Long Name

ATG12 Autophagy Related 12 Homolog

Alternate Names

APG12, FBR93, HAPG12

Entrez Gene IDs

9140 (Human); 67526 (Mouse); 361321 (Rat)

Gene Symbol

ATG12

UniProt

O94817

Additional ATG12 Products

Product Documents for Human/Mouse ATG12 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse ATG12 Antibody

For research use only

Related Research Areas

Autophagy

Citations for Human/Mouse ATG12 Antibody

Customer Reviews for Human/Mouse ATG12 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
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4 Stars
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1 Stars
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  • Human/Mouse ATG12 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 11/16/2017

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Protocols

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