Human/Mouse ATN1 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse ATN1 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ATN1
    Met1-Gln100
    Accession # P54259
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse ATN1 by Western Blot. Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse ATN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6567) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ATN1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
ATN1 in Human Brain. ATN1 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human/Mouse ATN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6567) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATN1

ATN1 (Atrophin-1; also DRPLA protein) is a 190‑200 kDa member of the Atrophin family of proteins that is cleaved into 120‑150 kDa fragments. ATN1 is a ubiquitously expressed transcriptional coactivator. Human ATN1 is 1185 amino acids (aa) in length. It contains multiple motifs, including an NLS (aa 16-32), interspersed poly-Pro, poly-Ser and poly-His regions (aa 376-707), two RE (ArgGlu) repeats (aa 816-934), and an NES (aa 1033-1042). There are at least 17 utilized phosphorylation sites and one acetylated Lys. ATN1 is most characterized by a poly-Gln region between aa 484-497. Normally, there are about 20 consecutive Gln residues, but this number may be increased to more than 70 in pathologic conditions. Proteolytic cleavage generates large C-terminal fragments of 120-150 kDa size. These are unlikely to contain the NLS, and thus are typically cytosolic. ATN1 is suggested to form heterodimers with full-length ATN2/RERE, thus generating a transcriptional repressor. There are multiple potential isoforms. One shows an alternative start site at Met527, while others differ in the number of glutamines in the poly-Gln region. Over aa 1-100, human ATN1 shares 94% aa identity with mouse ATN1.

  • Long Name:
    Atrophin 1
  • Entrez Gene IDs:
    1822 (Human); 13498 (Mouse); 29515 (Rat)
  • Alternate Names:
    ATN1; atrophin 1; atrophin-1; B37; D12S755E; dentatorubral-pallidoluysian atrophy (atrophin-1); Dentatorubral-pallidoluysian atrophy protein; DRPLA; DRPLANOD; HRS; NOD
Related Research Areas
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

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NorthernLights Guard Mounting Media

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VisUCyte Antigen Retrieval Reagent-Acidic

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VisUCyte Antigen Retrieval Reagent-Universal

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VisUCyte Antigen Retrieval Reagent-Basic

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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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