Human/Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Catalog # Availability Size / Price Qty
AF6664
AF6664-SP
Human/Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody in Western Blot
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Human/Mouse beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse beta -1,4-Glucuronyltransferase 1/B4GAT1 in Western blot. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) B4GalT1 and rhB3GNT 2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human beta -1,4-Glucuronyltransferase 1/B4GAT1
Asp43-Cys415
Accession # O43505
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse  beta -1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot. View Larger

Detection of Human and Mouse beta -1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot. Western blot shows lysates of human muscle tissue and mouse muscle tissue. PVDF Membrane was probed with 1 µg/mL of Human beta -1,4-Glucuronyltransferase 1/B4GAT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for beta -1,4-Glucuronyltransferase 1/B4GAT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,4-Glucuronyltransferase 1/B4GAT1

B3GNT1 ( beta -1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the beta 3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.

Long Name
beta-1,4-Glucuronyltransferase 1
Entrez Gene IDs
11041 (Human); 108902 (Mouse); 293667 (Rat)
Alternate Names
B3GNT1; B3GN-T1; B4GAT1; beta-1,3-N-acetylglucosaminyltransferase bGnT-6; BETA3GNTI; EC 2.4.1.149; i-beta-1,3-N-acetylglucosaminyltransferase; iGAT; iGNT; MDDGA13; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1B3GNT6; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6

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