Human/Mouse BID Antibody

Catalog # Availability Size / Price Qty
MAB860
MAB860-SP
Human/Mouse BID Antibody in Western Blot
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse BID Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse BID in Western blots. Detects full-length mouse BID and the 15 kDa carboxyl-terminal fragment (tBID) generated by cleavage with Caspase-8. The antibody also detects human BID. However, in Western blots of recombinant human and mouse BID, the antibody is most sensitive for mouse BID.
Source
Monoclonal Rat IgG1 Clone # 91508
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # P70444
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse BID by Western Blot. View Larger

Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of Recombinant Mouse BID and Recombinant Human BID untreated (-) or treated (+) with Caspase-8. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands for BID were detected at approximately 12 kDa and 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western Blot Detection of Human and Mouse BID by Western Blot. View Larger

Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BID

BID is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. BID is cytosolic in healthy cells but upon delivery of an apoptotic signal, BID is cleaved by Caspase-8. The cleaved form is translocated to the mitochondria outer membrane where it binds to BAK and the resulting complex causes altered mitochondrial membrane permeability.

Long Name
BH3 Interacting Domain Death Agonist
Entrez Gene IDs
637 (Human); 12122 (Mouse)
Alternate Names
apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID

Product Datasheets

Citations for Human/Mouse BID Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Impact of caspase-1/11, -3, -7, or IL-1?/IL-18 deficiency on rabies virus-induced macrophage cell death and onset of disease
    Authors: E Kip, F Nazé, V Suin, T Vanden Ber, A Francart, S Lamoral, P Vandenabee, R Beyaert, S Van Gucht, M Kalai
    Cell Death Discov, 2017;3(0):17012.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.
    Authors: Zhu, Wenhan, Tao, Lili, Quick, Marsha L, Joyce, Johanna, Qu, Jie-Ming, Luo, Zhao-Qin
    PLoS Pathog, 2015;11(3):e1004704.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo.
    Authors: Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C
    Cell Death Differ, 2014;21(9):1442-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. BH3-only protein Bid is dispensable for seizure-induced neuronal death and the associated nuclear accumulation of apoptosis-inducing factor.
    Authors: Engel T, Caballero-Caballero A, Schindler CK, Plesnila N, Strasser A, Prehn JH, Henshall DC
    J. Neurochem., 2010;115(1):92-101.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Activation of the extrinsic caspase pathway in cultured cortical neurons requires p53-mediated down-regulation of the X-linked inhibitor of apoptosis protein to induce apoptosis.
    Authors: Tun C, Guo W, Nguyen H, Yun B, Libby RT, Morrison RS, Garden GA
    J. Neurochem., 2007;102(4):1206-19.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Proteolysis of HIP during apoptosis occurs within a region similar to the BID loop.
    Authors: Caruso JA, Reiners JJ
    Apoptosis, 2006;11(11):1877-85.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot

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Mouse BID Antibody
By Anonymous on 05/19/2017
Application: WB Sample Tested: Mouse embryonic fibroblasts treated with TNFa (50 ng/ml) and cycloheximide (5 ug/ml) for 8 hours Species: Murine