Human/Mouse/Canine CD94 Antibody

  
  • Species Reactivity
    Human, Mouse, Canine
  • Specificity
    Detects recombinant human CD94 in direct ELISAs and human, mouse, and canine CD94 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human CD94
    Lys32-Ile179
    Accession # Q13241
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Canine CD94 by Western Blot. Western blot shows lysates of CLL‑1390 canine leukocytic round cell neoplasia cell line, HDLM human Hodgkin’s lymphoma cell line, and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD94 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD94 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD94

CD94 (also NK cell receptor, KLRD1 and KP43) is a 27‑31 kDa glycoprotein member of the C-type lectin superfamily. It is expressed on NK cells, particularly those that are CD56bright, plus those that are CD56dim but secrete material amounts of IFN-gamma. CD94 forms covalent transmembrane (TM) heterodimers with NKG2A, B, C, E and H, and binds HLA-E (in human; Qa-1b in mouse) as part of a cellular homeostatic monitoring system. Amide-linkage based complexes with NKG2A are predicted to run at 70-80 kDa in SDS-PAGE. Depending upon the exact NKG2 partner, CD94 ligation can result in either NK cell activation (2C, 2H or 2E) or inhibition (2A or 2B). Human CD94 is a 179 amino acid (aa) type II TM protein. It possesses a short cytoplasmic segment (aa 1-10) plus a 158 aa extracellular region (aa 32-179) that contains one C-type lectin domain (aa 61-176). Multiple splice variants exist. One is a 149 aa, 19 kDa cytosolic protein that shows an Ala substitution for aa 3-34, while a second is a truncated, 104 aa, 17 kDa TM protein. A third isoform is 158 aa in length and 25 kDa in size, and shows a deletion of aa 34-54. Over aa 32-179, human CD94 shares 53% aa identity with mouse CD94.

  • Entrez Gene IDs:
    3824 (Human); 16643 (Mouse)
  • Alternate Names:
    CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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