Human/Mouse CD44 Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse CD44 in flow cytometry.
  • Source
    Recombinant Monoclonal Rat IgG2B Clone # IM7.8.1R
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Myeloid leukemia M1 cells induced with Dexamethasone
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD44 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB6127R, filled histogram) or isotype control antibody (Catalog # IC013R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB6127R, filled histogram) or isotype control antibody (Catalog # IC013R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD44
CD44, also known as ECMR-III, Hermes Antigen, Pgp1, Ly-24, GP90, and HUTCH-I, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interaction through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagen, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44. In the ECD, human and mouse CD44 share about 65% aa sequence identity.
  • Entrez Gene IDs:
    960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
  • Alternate Names:
    CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC013R
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