Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # IM7.8.1R
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Myeloid leukemia M1 cells induced with Dexamethasone

Specificity

Detects human and mouse CD44 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse CD44 PE‑conjugated Antibody

Detection of CD44 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD44 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD44 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD44 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 PE‑conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse CD44 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMCs) and mouse splenocytes

Reviewed Applications

Read 1 review rated 5 using FAB6127P in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD44

CD44, also known as ECMR-III, Hermes Antigen, Pgp1, Ly-24, GP90, and HUTCH-I, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interaction through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagen, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44. In the ECD, human and mouse CD44 share about 65% aa sequence identity.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Gene Symbol

CD44

Additional CD44 Products

Product Documents for Human/Mouse CD44 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse CD44 PE‑conjugated Antibody

For research use only

Citations for Human/Mouse CD44 PE‑conjugated Antibody

Customer Reviews for Human/Mouse CD44 PE‑conjugated Antibody (1)

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  • Human/Mouse CD44 PE-conjugated Antibody
    Name: Tahmineh Safaie
    Application: Flow Cytometry
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 11/08/2019
    Human/Mouse CD44 PE‑conjugated Antibody FAB6127P

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