Detection of CD44 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE‑conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 PE‑conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD44, also known as
ECMR-III, Hermes Antigen, Pgp1, Ly-24, GP90, and HUTCH-I, is a ubiquitously expressed
protein that is the major receptor for hyaluronic acid (HA). CD44 mediates cell-cell
and cell-matrix interaction through its affinity for HA, and possibly also through
its affinity for other ligands such as osteopontin, collagen, and matrix
metalloproteinases (MMPs). Adhesion with HA plays an important role in cell
migration, tumor growth and progression. In cancer cells, may play an important
role in invadopodia formation. Also involved in lymphocyte activation,
recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal
sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding
disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa
transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large
number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has
been reported to recognize all isoforms of CD44. In the ECD, human and mouse CD44 share about 65% aa sequence identity.
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