Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Flow Cytometry, CyTOF-reported

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Complex Application

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG Clone # IM7.8.1R
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Myeloid leukemia M1 cells induced with Dexamethasone

Specificity

Detects human and mouse CD44 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG

Scientific Data Images for Human/Mouse CD44 Antibody

Detection of CD44 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD44 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Detection of CD44 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD44 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Applications for Human/Mouse CD44 Antibody

Application
Recommended Usage

CyTOF-reported

Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) and mouse splenocytes

Reviewed Applications

Read 1 review rated 5 using MAB6127 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD44

CD44, also known as ECMR-III, Hermes, Pgp1, Ly-24, GP90, or HUTCH, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Gene Symbol

CD44

Additional CD44 Products

Product Documents for Human/Mouse CD44 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse CD44 Antibody

For research use only

Citations for Human/Mouse CD44 Antibody

Customer Reviews for Human/Mouse CD44 Antibody (1)

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  • Human/Mouse CD44 Antibody
    Name: Tina Yuan
    Application: Immunohistochemistry
    Sample Tested: epithelium
    Species: Mouse
    Verified Customer | Posted 08/06/2023
    Human/Mouse CD44 Antibody MAB6127

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