Detection of CD44 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD44, also known as
ECMR-III, Hermes, Pgp1, Ly-24, GP90, or HUTCH, is a ubiquitously expressed
protein that is the major receptor for hyaluronic acid (HA). Mediates cell-cell
and cell-matrix interactions through its affinity for HA, and possibly also through
its affinity for other ligands such as osteopontin, collagens, and matrix
metalloproteinases (MMPs). Adhesion with HA plays an important role in cell
migration, tumor growth and progression. In cancer cells, may play an important
role in invadopodia formation. Also involved in lymphocyte activation,
recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal
sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding
disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa
transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large
number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has
been reported to recognize all isoforms of CD44.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.