Human/Mouse CELSR2 Antibody

(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human CELSR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CELSR2
    Cys51-Phe231
    Accession # Q9HCU4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CELSR2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR2. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CELSR2 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of CELSR2 in SH‑SY5Y Human Cell Line by Flow Cytometry.
SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Detection of CELSR2 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Immunohistochemistry
CELSR2 in Human Breast. CELSR2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to ductal epithelium. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CELSR2

CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2; also cadherin family member 10/CDHF10, Flamingo1 and EGFL2) is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa identity with mouse CELSR2.

  • Long Name:
    Cadherin, EGF LAG Seven-pass G-type Receptor 2
  • Entrez Gene IDs:
    1952 (Human); 53883 (Mouse); 83465 (Rat)
  • Alternate Names:
    cadherin EGF LAG seven-pass G-type receptor 2; Cadherin family member 10; cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila); CDHF10; CDHF10FLJ34118; CELSR2; EGFL2; EGFL2FLJ45143; EGF-like protein 2; EGF-like-domain, multiple 2; epidermal growth factor-like 2; Epidermal growth factor-like protein 2; Flamingo homolog 3; Flamingo1; FLJ45845; KIAA0279FLJ42737; MEGF3; MEGF3cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog; Multiple EGF-like domains protein 3; multiple epidermal growth factor-like domains 3; Multiple epidermal growth factor-like domains protein 3
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse CELSR2 Antibody AF6739
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Very Good
 WB Human Anonymous 05/03/2017
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Western Blot Human/Mouse CELSR2 Antibody AF6739
Western Blot: Human/Mouse CELSR2 Antibody [AF6739]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesHuman

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