Detection of CELSR2 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR2. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CELSR2 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of CELSR2 in SH‑SY5Y Human Cell Line by Flow Cytometry.
SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Detection of CELSR2 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
CELSR2 in Human Breast.
CELSR2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to ductal epithelium. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2; also cadherin family member 10/CDHF10, Flamingo1 and EGFL2) is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa identity with mouse CELSR2.
Cadherin, EGF LAG Seven-pass G-type Receptor 2
Entrez Gene IDs:
1952 (Human); 53883 (Mouse); 83465 (Rat)
cadherin EGF LAG seven-pass G-type receptor 2; Cadherin family member 10; cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila); CDHF10; CDHF10FLJ34118; CELSR2; EGFL2; EGFL2FLJ45143; EGF-like protein 2; EGF-like-domain, multiple 2; epidermal growth factor-like 2; Epidermal growth factor-like protein 2; Flamingo homolog 3; Flamingo1; FLJ45845; KIAA0279FLJ42737; MEGF3; MEGF3cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog; Multiple EGF-like domains protein 3; multiple epidermal growth factor-like domains 3; Multiple epidermal growth factor-like domains protein 3
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We have 1 review tested in 1 application: Western Blot.