Human/Mouse Cited-2 Antibody Summary
Accession # O35740
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse Cited‑2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cited-2 Monoclonal Antibody (Catalog # MAB5005) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cited-2 at approximately 30-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cited‑2 in NIH‑3T3 Mouse Cell Line. Cited-2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Human/Mouse Cited-2 Monoclonal Antibody (Catalog # MAB5005) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cited-2 is a 270 amino acid protein that functions by binding to the CH1 region of CREB binding protein (CBP)/p300. This binding interferes with certain transcription factors like HIF1 alpha and STAT2 blocking their ability to bind and interact with CBP/p300. Cited-2 knockout mice die due to defects in heart and neural tube development. Mutations in Cited-2 have been reported in patients with congenital heart defects. It appears Cited-2 modulates apoptosis by a p53 independent pathway whereby the details still need to be elucidated.
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