Human/Mouse COX4 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB6980AFP680

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 673803
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Product Specifications

Specificity

Detects human COX4-I2 in direct ELISAs. In Western blots, 100% cross-reactivity with recombinant human (rh) COX4-I1 andno cross-reactivity with rhCOX-1 or rhCOX-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human/Mouse COX4 Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: COX4

COX4-I2 (Cytochrome c oxidase subunit 4) is a 21-22 kDa member of the Cytochrome c oxidase IV family of proteins and is predominantly expressed in lung. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from Cytochrome c to dioxygen. COX4-I2 is induced following blockade of mitochondrial respiration, leading to increased production of reactive oxygen species and necrosis. The ubiquitously expressed COX4-I1 is the product of a different gene and shares only 50% aa identity with COX4-I2. Within aa 39-171 (the oxidase domain), human COX4-I2 shares 84% and 81% aa sequence identity with mouse and rat COX4-I2, respectively.

Long Name

Cytochrome c Oxidase Subunit IV

Alternate Names

COX4I2

Entrez Gene IDs

84701 (Human); 84682 (Mouse); 84683 (Rat)

Gene Symbol

COX4I2

UniProt

Additional COX4 Products

Product Documents for Human/Mouse COX4 Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse COX4 Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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