Human/Mouse COX4 Antibody

Catalog # Availability Size / Price Qty
MAB6980
MAB6980-SP
Detection of Human and Mouse COX4 by Western Blot.
3 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse COX4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human COX4-I2 in direct ELISAs. In Western blots, 100% cross-reactivity with recombinant human (rh) COX4-I1 and
no cross-reactivity with rhCOX-1 or rhCOX-2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 673803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human COX4-I2
Asn39-Lys171
Accession # Q96KJ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse COX4 antibody by Western Blot. View Larger

Detection of Human and Mouse COX4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX4 Monoclonal Antibody (Catalog # MAB6980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For addtional reference, recombinant human COX4-I1 (5 ng/lane) and recombinant human COX4-I2 (2.5 ng/lane) were included. A specific band was detected for COX4 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry COX4 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

COX4 in NIH‑3T3 Mouse Cell Line. COX4 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse COX4 Monoclonal Antibody (Catalog # MAB6980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry COX4 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

COX4 in HeLa Human Cell Line. COX4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse COX4 Monoclonal Antibody (Catalog # MAB6980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COX4

COX4-I2 (Cytochrome c oxidase subunit 4) is a 21-22 kDa member of the Cytochrome c oxidase IV family of proteins and is predominantly expressed in lung. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from Cytochrome c to dioxygen. COX4-I2 is induced following blockade of mitochondrial respiration, leading to increased production of reactive oxygen species and necrosis. The ubiquitously expressed COX4-I1 is the product of a different gene and shares only 50% aa identity with COX4-I2. Within aa 39-171 (the oxidase domain), human COX4-I2 shares 84% and 81% aa sequence identity with mouse and rat COX4-I2, respectively.

Long Name
Cytochrome c Oxidase Subunit IV
Entrez Gene IDs
84701 (Human); 84682 (Mouse); 84683 (Rat)
Alternate Names
COX IV-1; COX4; COX4-1; COX4COXIV; COX4I2; Cytochrome c oxidase polypeptide IV; cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV; MGC72016

Product Datasheets

Citations for Human/Mouse COX4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors
    Authors: X Ma, F Cheng, K Yuan, K Jiang, T Zhu
    Mol Med Rep, 2019;20(2):879-886.
    Species: Human
    Sample Types: Cell Lyates
    Applications: Western Blot
  2. SIRT3 diminishes inflammation and mitigates endotoxin-induced acute lung injury
    Authors: D Kurundkar, AR Kurundkar, NB Bone, EJ Becker, W Liu, B Chacko, V Darley-Usm, JW Zmijewski, VJ Thannickal
    JCI Insight, 2019;4(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Frataxin-deficient neurons and mice models of Friedreich ataxia are improved by TAT-MTScs-FXN treatment
    Authors: E Britti, F Delaspre, A Feldman, M Osborne, H Greif, J Tamarit, J Ros
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse COX4 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse COX4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse COX4 Antibody
By Anonymous on 12/06/2021
Application: WB Sample Tested: Liver cells Species: Mouse