Detection of Human and Mouse DAPP1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DAPP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7024) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DAPP1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DAPP1 (Dual Adaptor for Phosphotyrosine and 3-Phosphoinosidides 1; also Bam32 and PHISH) is a 31-32 kDa member of Ig-superfamily of proteins. It shows restricted expression, being found in mast cells, dendritic cells, and germinal center B cells. With respect to B cells, and upon B cell receptor engagement, PI3-kinase is activated, resulting in the generation of membrane-embedded PI(3,4)P2. This product serves as a ligand for cytosolic DAPP1, resulting in its immobilization at the cell membrane. Here, it is phosphorylated on Tyr139, directly regulating HPK1 (hematopoietic progenitor kinase 1) activity, and indirectly regulation HPK1 downstream targets ERK and JNK. Functionally, DAPP1 plays a role in BCR internalization, antibody isotype switching, antigen processing and presentation, and B cell survival. Human DAPP1 is 280 amino acids (aa) in length. It contains one SH2 domain (aa 35‑129), a utilized phosphorylation site at Tyr139 and a C-terminal PH domain (aa 164‑259). When phosphorylated, the observed MW of DAPP1 may be increased by 2-4 kDa in SDS-PAGE. There are four potential alternative splice variants. Two contain a five and 22 aa substitution for aa 259-280, respectively, while a third possesses a 14 aa substitution for aa 1-229, and a fourth shows deletions of aa 35‑75 and aa 180-200 coupled to a three aa substitution for aa 249-280. Over aa 1-163, human DAPP1 shares 91% aa sequence identity with mouse DAPP1.
Dual Adaptor of Phosphotyrosine and 3-Phosphoinositides 1
Entrez Gene IDs:
27071 (Human); 26377 (Mouse); 362046 (Rat)
B lymphocyte adapter protein Bam32; BAM32; BAM32DKFZp667E0716; B-cell adapter molecule of 32 kDa; DAPP1; dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; dual adaptor of phosphotyrosine and 3-phosphoinositides; hDAPP1
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