|Detection of Mouse DCBLD2/ESDN by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCBLD2/ESDN at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
|Detection of DCBLD2/ESDN in NCI-H460 Human Cell Line by Flow Cytometry. NCI‑H460 human large cell lung carcinoma cell line was stained with Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).|
DCBLD2 (discoidin, CUB, and LCCL domain containing 2), also known as ESDN (endothelial and smooth muscle cell derived neuropilin-like) and CLCP1, was identified through its up-regulation in a highly metastatic lung cancer cell line. DCBLD2 has structural similarities to neuropilins, VEGF receptors, and semaphorins, and is thought to play a role in cell motility and metastasis. SEMA4B is a receptor for DCBLD2/ESDN/CLCP1 and this interaction may be involved in regulating cancer cell metastasis.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided