Human/Mouse Desmin Antibody

Catalog # Availability Size / Price Qty
AF3844
AF3844-SP

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Desmin in Human Heart.
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Product Details
Citations (16)
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Human/Mouse Desmin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Desmin.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Desmin
Val261-Leu470
Accession # P17661
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Desmin antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

Desmin in Human Heart. Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Desmin antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr). View Larger

Desmin in Mouse Skeletal Muscle. Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmin

Desmin is a 53 kDa, muscle-specific molecule that belongs to the intermediate filament superfamily of cytoskeletal proteins. Cytoplasmic Desmin homopolymers form stable filamentous networks that stabilize lateral alignments of myofibrils. Human Desmin is 470 amino acid (aa) peptide. It contains an N-terminal head segment (aa 1-84), multiple coiled regions (aa 109-412), and a tail region (aa 413-470). Single amino acid substitutions and three-amino acid deletions are associated with cardio‑skeletal myopathies. Most mutations affect filament assembly. Desmin is a highly conserved protein. Over the region used as immunogen, human Desmin is 99% identical to the corresponding mouse, canine, and porcine protein sequences.

Entrez Gene IDs
1674 (Human); 13346 (Mouse); 64362 (Rat)
Alternate Names
CMD1I; CMD1IFLJ41013; CSM1; CSM2; DES; Desmin; FLJ12025; FLJ39719; FLJ41793; intermediate filament protein

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Citations for Human/Mouse Desmin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. The Desmin Mutation DES-c.735G>C Causes Severe Restrictive Cardiomyopathy by Inducing In-Frame Skipping of Exon-3
    Authors: A Brodehl, C Hain, F Flottmann, S Ratnavadiv, A Gaertner, B Klauke, J Kalinowski, H Körperich, J Gummert, L Paluszkiew, MA Deutsch, H Milting
    Biomedicines, 2021;9(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape
    Authors: LE Bruijn, BEWM van den Ak, CM van Rhijn, JF Hamming, JHN Lindeman
    J Am Heart Assoc, 2020;9(23):e017094.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Inhibition of aquaporin-3 in macrophages by a monoclonal antibody as potential therapy for liver injury
    Authors: M Hara-Chiku, M Tanaka, AS Verkman, M Yasui
    Nat Commun, 2020;11(1):5666.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Lineage Tracing Reveals the Bipotency of SOX9+ Hepatocytes during Liver Regeneration
    Authors: X Han, Y Wang, W Pu, X Huang, L Qiu, Y Li, W Yu, H Zhao, X Liu, L He, L Zhang, Y Ji, J Lu, KO Lui, B Zhou
    Stem Cell Reports, 2019;12(3):624-638.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Tumor stroma-targeted antibody-drug conjugate triggers localized anticancer drug release
    Authors: C Szot, S Saha, XM Zhang, Z Zhu, MB Hilton, K Morris, S Seaman, JM Dunleavey, KS Hsu, GJ Yu, H Morris, DA Swing, DC Haines, Y Wang, J Hwang, Y Feng, D Welsch, G DeCrescenz, A Chaudhary, E Zudaire, DS Dimitrov, B St Croix
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Altered AKAP12 expression in portal fibroblasts and liver sinusoids mediates transition from hepatic fibrogenesis to fibrosis resolution
    Authors: HS Lee, J Choi, T Son, HJ Wee, SJ Bae, JH Seo, JH Park, SH Ryu, D Lee, MK Jang, E Yu, YH Chung, KW Kim
    Exp. Mol. Med., 2018;50(4):48.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy
    Authors: A Dubrac, SE Künzel, SH Künzel, J Li, RR Chandran, K Martin, DM Greif, RH Adams, A Eichmann
    Nat Commun, 2018;9(1):3463.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Aneurysm Severity is Increased by Combined Mmp-7 Deletion and N-cadherin Mimetic (EC4-Fc) Over-Expression
    Authors: CA Lyon, H Williams, R Bianco, SJ Simmonds, BA Brown, KS Wadey, FCT Smith, JL Johnson, SJ George
    Sci Rep, 2017;7(1):17342.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages
    Authors: TE Peterson, ND Kirkpatric, Y Huang, CT Farrar, KA Marijt, J Kloepper, M Datta, Z Amoozgar, G Seano, K Jung, WS Kamoun, T Vardam, M Snuderl, J Goveia, S Chatterjee, A Batista, A Muzikansky, CC Leow, L Xu, TT Batchelor, DG Duda, D Fukumura, RK Jain
    Proc Natl Acad Sci USA, 2016;113(16):4470-5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival
    Authors: J Kloepper, L Riedemann, Z Amoozgar, G Seano, K Susek, V Yu, N Dalvie, RL Amelung, M Datta, JW Song, V Askoxylaki, JW Taylor, C Lu-Emerson, A Batista, ND Kirkpatric, K Jung, M Snuderl, A Muzikansky, KG Stubenrauc, O Krieter, H Wakimoto, L Xu, LL Munn
    Proc Natl Acad Sci USA, 2016;113(16):4476-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
    Authors: Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
    Nat Biotechnol, 2015;33(1):89-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  12. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015;10(7):e0128975.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.
    Authors: Durruthy-Durruthy, Jens, Briggs, Sharon F, Awe, Jason, Ramathal, Cyril Y, Karumbayaram, Saravana, Lee, Patrick, Heidmann, Julia D, Clark, Amander, Karakikes, Ioannis, Loh, Kyle M, Wu, Joseph C, Hoffman, Andrew R, Byrne, James, Reijo Pera, Renee A, Sebastiano, Vittorio
    PLoS ONE, 2014;9(4):e94231.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death.
    Authors: Brodehl A, Dieding M, Klauke B, Dec E, Madaan S, Huang T, Gargus J, Fatima A, Saric T, Cakar H, Walhorn V, Tonsing K, Skrzipczyk T, Cebulla R, Gerdes D, Schulz U, Gummert J, Svendsen J, Olesen M, Anselmetti D, Christensen A, Kimonis V, Milting H
    Circ Cardiovasc Genet, 2013;6(6):615-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  15. Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants.
    Authors: Brodehl A, Hedde PN, Dieding M, Fatima A, Walhorn V, Gayda S, Šarić T, Klauke B, Gummert J, Anselmetti D, Heilemann M, Nienhaus GU, Milting H
    J. Biol. Chem., 2012;287(19):16047-57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
    Authors: Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B
    Cell Res, 0;26(1):119-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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