Human/Mouse Desmin Antibody

Catalog # Availability Size / Price Qty
AF3844
AF3844-SP
Desmin in Human Heart.
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Product Details
Citations (30)
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Human/Mouse Desmin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Desmin.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Desmin
Val261-Leu470
Accession # P17661
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Desmin antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

Desmin in Human Heart. Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Desmin antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr). View Larger

Desmin in Mouse Skeletal Muscle. Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Human Desmin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Desmin by Immunocytochemistry/Immunofluorescence Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Desmin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Desmin by Immunocytochemistry/Immunofluorescence In vitro and in vivo differentiation of GMP-transitioned RiPSC lines.(A) Directed in vitro differentiation of three GMP-transitioned RiPSC lines into three germ layers followed by FACS analysis (endoderm) and immunocytochemistry (ectoderm, mesoderm). Scale bar = 150 μm. (B) Comparison of differentiation potential into neuroectoderm (PAX6) and mesoderm (DESMIN) between non-GMP (research-grade) and GMP-grade lines (BJ, HUF1). Comparisons are based on counting analysis of cells that were positive for the specific differentiation markers. N = 200–300. (C) Hematoxylin and eosin staining of teratomas derived from GMP-transitioned lines showing ectoderm (neural rosettes, epidermis), mesoderm (cartilage) and endoderm (gut-like endothelium). Scale bar = 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24718618), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmin

Desmin is a 53 kDa, muscle-specific molecule that belongs to the intermediate filament superfamily of cytoskeletal proteins. Cytoplasmic Desmin homopolymers form stable filamentous networks that stabilize lateral alignments of myofibrils. Human Desmin is 470 amino acid (aa) peptide. It contains an N-terminal head segment (aa 1-84), multiple coiled regions (aa 109-412), and a tail region (aa 413-470). Single amino acid substitutions and three-amino acid deletions are associated with cardio‑skeletal myopathies. Most mutations affect filament assembly. Desmin is a highly conserved protein. Over the region used as immunogen, human Desmin is 99% identical to the corresponding mouse, canine, and porcine protein sequences.

Entrez Gene IDs
1674 (Human); 13346 (Mouse); 64362 (Rat)
Alternate Names
CMD1I; CMD1IFLJ41013; CSM1; CSM2; DES; Desmin; FLJ12025; FLJ39719; FLJ41793; intermediate filament protein

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Citations for Human/Mouse Desmin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Combined Treatment with Acalabrutinib and Rapamycin Inhibits Glioma Stem Cells and Promotes Vascular Normalization by Downregulating BTK/mTOR/VEGF Signaling
    Authors: Yu-Kai Su, Oluwaseun Adebayo Bamodu, I-Chang Su, Narpati Wesa Pikatan, Iat-Hang Fong, Wei-Hwa Lee et al.
    Pharmaceuticals (Basel)
  2. Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model
    Authors: Ludovica Ceci, Lixian Chen, Leonardo Baiocchi, Nan Wu, Lindsey Kennedy, Guido Carpino et al.
    Cellular and Molecular Gastroenterology and Hepatology
  3. Neuropilin-1 and platelet-derived growth factor receptors cooperatively regulate intermediate filaments and mesenchymal cell migration during alveolar septation
    Authors: Stephen E. McGowan, Diann M. McCoy
    American Journal of Physiology-Lung Cellular and Molecular Physiology
  4. A combined human gastruloid model of cardiogenesis and neurogenesis
    Authors: Olmsted ZT, Paluh JL.
    iScience
  5. Downregulation of syndecan?1 expression induces activation of hepatic stellate cells via the TGF??1/Smad3 signaling pathway
    Authors: M Deng, L Xu, X Xie, X Sheng, Z Zou, M Yao
    Mol Med Rep, 2019-05-09;0(0):.
  6. Chemerin regulates normal angiogenesis and hypoxia-driven neovascularization
    Authors: Cyrine Ben Dhaou, Kamel Mandi, Mickaël Frye, Angela Acheampong, Ayoub Radi, Benjamin De Becker et al.
    Angiogenesis
  7. Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity
    Authors: Lauren B. Rodda, Erick Lu, Mariko L. Bennett, Caroline L. Sokol, Xiaoming Wang, Sanjiv A. Luther et al.
    Immunity
  8. Targeting Tumor Vascular CD99 Inhibits Tumor Growth
    Authors: Elisabeth J. M. Huijbers, Inge M. van der Werf, Lisette D. Faber, Lena D. Sialino, Pia van der Laan, Hanna A. Holland et al.
    Frontiers in Immunology
  9. Long-term and potent IOP-lowering effect of I kappa B alpha -siRNA in a nonhuman primate model of chronic ocular hypertension
    Authors: Difang Sun, Zongyi Zhan, Rui Zeng, Xiaolin Liu, Bin Wang, Fan Yang et al.
    iScience
  10. Hepatic stellate and endothelial cells maintain hematopoietic stem cells in the developing liver
    Authors: Yeojin Lee, Juliana Leslie, Ying Yang, Lei Ding
    Journal of Experimental Medicine
  11. Spatial-temporal proliferation of hepatocytes during pregnancy revealed by genetic lineage tracing
    Authors: He, S;Guo, Z;Zhou, M;Wang, H;Zhang, Z;Shi, M;Li, X;Yang, X;He, L;
    Cell stem cell
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. The Desmin Mutation DES-c.735G>C Causes Severe Restrictive Cardiomyopathy by Inducing In-Frame Skipping of Exon-3
    Authors: A Brodehl, C Hain, F Flottmann, S Ratnavadiv, A Gaertner, B Klauke, J Kalinowski, H Körperich, J Gummert, L Paluszkiew, MA Deutsch, H Milting
    Biomedicines, 2021-10-05;9(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape
    Authors: LE Bruijn, BEWM van den Ak, CM van Rhijn, JF Hamming, JHN Lindeman
    J Am Heart Assoc, 2020-11-16;9(23):e017094.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Inhibition of aquaporin-3 in macrophages by a monoclonal antibody as potential therapy for liver injury
    Authors: M Hara-Chiku, M Tanaka, AS Verkman, M Yasui
    Nat Commun, 2020-11-09;11(1):5666.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Lineage Tracing Reveals the Bipotency of SOX9+ Hepatocytes during Liver Regeneration
    Authors: X Han, Y Wang, W Pu, X Huang, L Qiu, Y Li, W Yu, H Zhao, X Liu, L He, L Zhang, Y Ji, J Lu, KO Lui, B Zhou
    Stem Cell Reports, 2019-02-14;12(3):624-638.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy
    Authors: A Dubrac, SE Künzel, SH Künzel, J Li, RR Chandran, K Martin, DM Greif, RH Adams, A Eichmann
    Nat Commun, 2018-08-27;9(1):3463.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Tumor stroma-targeted antibody-drug conjugate triggers localized anticancer drug release
    Authors: C Szot, S Saha, XM Zhang, Z Zhu, MB Hilton, K Morris, S Seaman, JM Dunleavey, KS Hsu, GJ Yu, H Morris, DA Swing, DC Haines, Y Wang, J Hwang, Y Feng, D Welsch, G DeCrescenz, A Chaudhary, E Zudaire, DS Dimitrov, B St Croix
    J. Clin. Invest., 2018-06-04;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Altered AKAP12 expression in portal fibroblasts and liver sinusoids mediates transition from hepatic fibrogenesis to fibrosis resolution
    Authors: HS Lee, J Choi, T Son, HJ Wee, SJ Bae, JH Seo, JH Park, SH Ryu, D Lee, MK Jang, E Yu, YH Chung, KW Kim
    Exp. Mol. Med., 2018-04-27;50(4):48.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Aneurysm Severity is Increased by Combined Mmp-7 Deletion and N-cadherin Mimetic (EC4-Fc) Over-Expression
    Authors: CA Lyon, H Williams, R Bianco, SJ Simmonds, BA Brown, KS Wadey, FCT Smith, JL Johnson, SJ George
    Sci Rep, 2017-12-11;7(1):17342.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival
    Authors: J Kloepper, L Riedemann, Z Amoozgar, G Seano, K Susek, V Yu, N Dalvie, RL Amelung, M Datta, JW Song, V Askoxylaki, JW Taylor, C Lu-Emerson, A Batista, ND Kirkpatric, K Jung, M Snuderl, A Muzikansky, KG Stubenrauc, O Krieter, H Wakimoto, L Xu, LL Munn
    Proc Natl Acad Sci USA, 2016-04-04;113(16):4476-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages
    Authors: TE Peterson, ND Kirkpatric, Y Huang, CT Farrar, KA Marijt, J Kloepper, M Datta, Z Amoozgar, G Seano, K Jung, WS Kamoun, T Vardam, M Snuderl, J Goveia, S Chatterjee, A Batista, A Muzikansky, CC Leow, L Xu, TT Batchelor, DG Duda, D Fukumura, RK Jain
    Proc Natl Acad Sci USA, 2016-04-04;113(16):4470-5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
    Authors: Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B
    Cell Res, 2015-12-04;26(1):119-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015-07-15;10(7):e0128975.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
    Authors: Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
    Nat Biotechnol, 2014-11-10;33(1):89-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  25. Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.
    Authors: Durruthy-Durruthy, Jens, Briggs, Sharon F, Awe, Jason, Ramathal, Cyril Y, Karumbayaram, Saravana, Lee, Patrick, Heidmann, Julia D, Clark, Amander, Karakikes, Ioannis, Loh, Kyle M, Wu, Joseph C, Hoffman, Andrew R, Byrne, James, Reijo Pera, Renee A, Sebastiano, Vittorio
    PLoS ONE, 2014-04-09;9(4):e94231.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death.
    Authors: Brodehl A, Dieding M, Klauke B, Dec E, Madaan S, Huang T, Gargus J, Fatima A, Saric T, Cakar H, Walhorn V, Tonsing K, Skrzipczyk T, Cebulla R, Gerdes D, Schulz U, Gummert J, Svendsen J, Olesen M, Anselmetti D, Christensen A, Kimonis V, Milting H
    Circ Cardiovasc Genet, 2013-11-07;6(6):615-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  27. Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants.
    Authors: Brodehl A, Hedde PN, Dieding M, Fatima A, Walhorn V, Gayda S, Šarić T, Klauke B, Gummert J, Anselmetti D, Heilemann M, Nienhaus GU, Milting H
    J. Biol. Chem., 2012-03-08;287(19):16047-57.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  28. Microtubule associated protein 4 phosphorylation-induced epithelial-to-mesenchymal transition of podocyte leads to proteinuria in diabetic nephropathy
    Authors: Lingfei Li, Yanhai Feng, Junhui Zhang, Qiong Zhang, Jun Ren, Cheng Sun et al.
    Cell Communication and Signaling
  29. Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells
    Authors: Anitha K. Shenoy, Yue Jin, Huacheng Luo, Ming Tang, Christine Pampo, Rong Shao et al.
    Journal of Clinical Investigation
  30. Cocaine-induced release of CXCL10 from pericytes regulates monocyte transmigration into the CNS.
    Authors: Niu F, Liao K, Hu G et al.
    J. Cell Biol.

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