Detects human and mouse FGF-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) FGF-5, rhFGF-7, and rhFGF-9 is observed.
Monoclonal Mouse IgG1 Clone # 47109
Protein A or G purified from ascites
E. coli-derived recombinant mouse FGF-8b Gln23-Arg215 Accession # NP_001159834
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human FGF-8a (Catalog # 4745-F8) Recombinant Mouse FG-8b (Catalog # 423-F8) Recombinant Mouse FGF-8c (Catalog # 424-FC) Recombinant Human FGF-8e (Catalog # 4746-F8) Recombinant Human FGF-8f (Catalog # 5027-FF)
Measured by its ability to neutralize FGF‑8-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.25-0.75 µg/mL in the presence of 125 ng/mL Recombinant Mouse FGF‑8 c Isoform and 0.1 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑8 and Neutralization by Human and Mouse FGF‑8 Antibody.
Recombinant Mouse FGF‑8 c Isoform (Catalog # 424-FC) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF‑8 c Isoform (125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse FGF‑8 Monoclonal Antibody (Catalog # MAB323). The ND50 is typically 0.25-0.75 µg/mL in the presence of heparin (0.1 µg/mL).
FGF‑8 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Mouse FGF‑8 Monoclonal Antibody (Catalog # MAB323) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
is a member of the fibroblast growth factor family that was originally
discovered as a growth factor essential for the androgen-dependent
growth of mouse mammary carcinoma cells (1-3). Alternate splicing of
mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but
only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino
acid (aa) signal sequence, an N‑terminal domain that varies according to
the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a
125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF
domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18
(67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100%
aa identity with human FGF-8b. FGF-8 is widely expressed
during embryogenesis, and mediates epithelial-mesenchymal transitions.
It plays an organizing and inducing role during gastrulation, and
regulates patterning of the midbrain/hindbrain, eye, ear, limbs and
heart in the embryo (2, 5 - 8). The isoforms may play different roles in
development. FGF-8b shows the strongest receptor affinity and oncogenic
transforming capacity although FGF-8a and FGF-8e are also transforming
and have been found in human prostate, breast or ovarian tumors
(1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but
low levels are found in the reproductive and genitourinary tract,
peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.