Human/Mouse FGF-8 Antibody Summary
Accession # NP_001159834
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑8 and Neutralization by Human and Mouse FGF‑8 Antibody. Recombinant Mouse FGF-8 c Isoform (Catalog # 424-FC) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF-8 c Isoform (125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse FGF-8 Monoclonal Antibody (Catalog # MAB323). The ND50 is typically 0.25-0.75 µg/mL in the presence of heparin (0.1 µg/mL).
FGF‑8 in Human Prostate. FGF-8 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Mouse FGF-8 Monoclonal Antibody (Catalog # MAB323) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).
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- Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
- Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
- Gemel, J. et al. (1996) Genomics 35:253.
- Olsen, S.K. et al. (2006) Genes Dev. 20:185.
- Crossley, P.H. et al. (1996) Cell, 84:127.
- Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
- Sun, X. et al. (1999) Genes Dev. 13:1834.
- Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
- Mattila, M.M. et al. (2001) Oncogene 20:2791.
- Valve, E. et al. (2000) Int. J. Cancer 88:718.
- Valve, E.M. et al. (2001) Lab. Invest. 81:815.
- Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.
Citations for Human/Mouse FGF-8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions
Authors: P Nowialis, K Lopusna, J Opavska, SL Haney, A Abraham, P Sheng, A Riva, A Natarajan, O Guryanova, M Simpson, R Hlady, M Xie, R Opavsky
Nat Commun, 2019-09-26;10(1):4374.
Sample Types: Tissue Lysates
Applications: Western Blot
Specificity protein 7 is required for proliferation and differentiation of ameloblasts and odontoblasts
Authors: JM Bae, JC Clarke, H Rashid, MD Adhami, K McCullough, JS Scott, H Chen, KM Sinha, B de Crombru, A Javed
J. Bone Miner. Res., 2018-03-24;0(0):.
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: Effects on Embryo Survival
J Biol Chem, 2016-07-08;0(0):.
Sample Types: Protein
Applications: Western Blot
Local apoptosis modulates early mammalian brain development through the elimination of morphogen-producing cells.
Authors: Nonomura K, Yamaguchi Y, Hamachi M, Koike M, Uchiyama Y, Nakazato K, Mochizuki A, Sakaue-Sawano A, Miyawaki A, Yoshida H, Kuida K, Miura M
Dev Cell, 2013-12-23;27(6):621-34.
Sample Types: Whole Tissue
Cell aggregation-induced FGF8 elevation is essential for P19 cell neural differentiation.
Authors: Wang C, Xia C, Bian W, Liu L, Lin W, Chen YG, Ang SL, Jing N
Mol. Biol. Cell, 2006-04-26;17(7):3075-84.
Sample Types: Whole Cells
Regulation of avian cardiogenesis by Fgf8 signaling.
Authors: Alsan BH, 2019, Schultheiss TM
Sample Types: In Ovo
Applications: In Ovo
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