Human/Mouse Frizzled-3 Alexa Fluor® 647-conjugated Antibody

FAB1001R Datasheet / COA / SDS

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FAB1001R-100UG

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Human/Mouse Frizzled-3 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -4, -7, or -8 is observed. By flow cytometry and immunohistochemistry, this antibody also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. This antibody also detects Frizzled-3 on human HEK293 cells by immunocytochemistry and flow cytometry.

Source

Monoclonal Rat IgG2a Clone # 169310

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Met1-Val157
Accession # Q61086

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

HEK293 human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Frizzled-3

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Entrez Gene IDs

7976 (Human); 14365 (Mouse)

Alternate Names

frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3