Human/Mouse Frizzled-3 Alexa Fluor® 750-conjugated Antibody

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R&D Systems Antibodies
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Human/Mouse Frizzled-3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -4, -7, or -8 is observed. By flow cytometry and immunohistochemistry, this antibody also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. This antibody also detects Frizzled-3 on human HEK293 cells by immunocytochemistry and flow cytometry.
Monoclonal Rat IgG2a Clone # 169310
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Accession # Q61086
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
HEK293 human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Frizzled-3

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Entrez Gene IDs
7976 (Human); 14365 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3

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