|GLI‑1 in A172 Human Cell Line. GLI‑1 was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Human/Mouse GLI‑1 Monoclonal Antibody (Catalog # MAB3324) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti‑Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
|Detection of Human/Mouse GLI‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse GLI‑1 Monoclonal Antibody (Catalog # MAB3324) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for GLI‑1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI- activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.
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