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Human/Mouse GLI-1 Antibody

Catalog #: MAB3324
4 Citations 1 Review Datasheet / COA / SDS

Discontinued Product

MAB3324 has been discontinued.
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GLI‑1 in A172 Human Cell Line.
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Product Details
Citations (4)
FAQs
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GLI-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse GLI-1.
Source
Monoclonal Rat IgG2A Clone # 388516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GLI-1
Met1-Glu234
Accession # P08151
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL

Recombinant Human GLI‑1

Recombinant Mouse GLI‑1

Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry GLI‑1 antibody in A172 Human Cell Line by Immunocytochemistry (ICC). View Larger

GLI‑1 in A172 Human Cell Line. GLI-1 was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Human/Mouse GLI-1 Monoclonal Antibody (Catalog # MAB3324) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-1

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI- activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Entrez Gene IDs
2735 (Human); 14632 (Mouse); 140589 (Rat)
Alternate Names
GLI family zinc finger 1; GLI1; GLI-1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Glioma-associated oncogene; GLIzinc finger protein GLI1; Oncogene GLI

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Citations for Human/Mouse GLI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Gli1 Expression in Human Epiretinal Membranes
    Authors: S Jeon, J Baek, WK Lee
    Invest. Ophthalmol. Vis. Sci, 2017-01-01;58(1):651-659.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis.
    Authors: Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang S, Rennke H, Waikar S, Humphreys B
    J Clin Invest, 2015-07-20;125(8):2935-51.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. beta-Catenin-Gli1 interaction regulates proliferation and tumor growth in medulloblastoma.
    Authors: Zinke J, Schneider F, Harter P, Thom S, Ziegler N, Toftgard R, Plate K, Liebner S
    Mol Cancer, 2015-02-03;14(0):17.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  4. Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway.
    Authors: Yaddanapudi K, De Miranda J, Hornig M
    PLoS ONE, 2011-10-25;6(10):e26766.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Human/Mouse GLI-1 Antibody

Average Rating: 3 (Based on 1 Review)

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Human/Mouse GLI-1 Antibody
By Anonymous on 12/15/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: perivascular stem cells Species: Mouse

We are using this product to study perivascular stem cells in human and mouse organs. We were pleased to find positive staining in pericytes of human frozen foetal tissue and paraffin embedded mouse tissue, as pervious publications seemed unable to get satisfactory immunological staining in tissues.