hh Human/Mouse GLI-1 Antibody: R&D Systems

Human/Mouse GLI-1 Antibody

MAB3324 has been discontinued. View all GLI-1 products.
(4 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects endogenous human and mouse GLI-1.
  • Source
    Monoclonal Rat IgG2A Clone # 388516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human GLI-1
    Met1-Glu234
    Accession # P08151
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL

    Recombinant Human GLI‑1

    Recombinant Mouse GLI‑1

  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
GLI‑1 in A172 Human Cell Line. GLI‑1 was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Human/Mouse GLI‑1 Monoclonal Antibody (Catalog # MAB3324) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti‑Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLI-1

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI- activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

  • Long Name:
    Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
  • Entrez Gene IDs:
    2735 (Human); 14632 (Mouse); 140589 (Rat)
  • Alternate Names:
    GLI family zinc finger 1; GLI1; GLI-1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Glioma-associated oncogene; GLIzinc finger protein GLI1; Oncogene GLI
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Gli1 Expression in Human Epiretinal Membranes
    Authors: S Jeon, J Baek, WK Lee
    Invest. Ophthalmol. Vis. Sci, 2017;58(1):651-659.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. beta-Catenin-Gli1 interaction regulates proliferation and tumor growth in medulloblastoma.
    Authors: Zinke J, Schneider F, Harter P, Thom S, Ziegler N, Toftgard R, Plate K, Liebner S
    Mol Cancer, 2015;14(0):17.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis.
    Authors: Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang S, Rennke H, Waikar S, Humphreys B
    J Clin Invest, 2015;125(8):2935-51.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway.
    Authors: Yaddanapudi K, De Miranda J, Hornig M
    PLoS ONE, 2011;6(10):e26766.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
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 ICC/IF Mouse Anonymous 12/15/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedperivascular stem cells
SpeciesMouse

Other Experimental Details

Other Experimental DetailsWe are using this product to study perivascular stem cells in human and mouse organs. We were pleased to find positive staining in pericytes of human frozen foetal tissue and paraffin embedded mouse tissue, as pervious publications seemed unable to get satisfactory immunological staining in tissues.

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