Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all HMGA1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HMGA1B
Met1-Glu96
Accession # P17096

Specificity

Detects endogenous human and mouse HMGA1B in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse HMGA1B Antibody

Detection of Human/Mouse HMGA1B antibody by Western Blot.

Detection of Human/Mouse HMGA1B by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HMGA1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5956) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HMGA1B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse HMGA1B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using AF5956 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HMGA1B

HMGA1B (High mobility group A1, isoform B; also HMG-Y) is a 10‑11 kDa member of the HMGA family of proteins. It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue. HMGA1B is a nonhistone architectural protein that binds to AT-rich DNA sequences. It participates in both gene repression and activation by changing DNA conformation, and is known to regulate miRNA as well. Human HMGA1B is 96 amino acids (aa) in length and contains three A-T hook DNA-binding domains (aa 21‑31; 42‑52; 67‑78). It is constitutively phosphorylated, and may undergo acetylation. There are three potential splice variants. All three show the same 11 aa insertion after Pro34. In addition, one form shows an additional 56 aa substitution for aa 55‑96, while a second shows a 14 aa substitution for aa 80‑96. Full-length human HMGA1B shares 97% aa identity with mouse HMGA1B.

Long Name

High Mobility Group AT-hook 1B

Alternate Names

HMG-R, HMG-Y, HMGA1, HMGIY

Entrez Gene IDs

3159 (Human); 15361 (Mouse); 117062 (Rat)

Gene Symbol

HMGA1

UniProt

P17096

Additional HMGA1B Products

Product Documents for Human/Mouse HMGA1B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse HMGA1B Antibody

For research use only

Citations for Human/Mouse HMGA1B Antibody

Customer Reviews for Human/Mouse HMGA1B Antibody (1)

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  • Human/Mouse HMGA1B Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Pancreatic cancer cells
    Species: Human
    Verified Customer | Posted 01/17/2018
    Human/Mouse HMGA1B Antibody AF5956

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