Human/Mouse IkB‑ beta Antibody

Name: Human/Mouse IkB‑ beta Antibody
Brand: R&D Systems
SKU: AF5225

R&D Systems | Catalog # AF5225

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Citations (3)

Product Documents

Discontinued Product

AF5225 has been discontinued. View all IkB-beta products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IkB-beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse I kappa B-beta
Met1-Ala359
Accession # Q60778

Specificity

Detects endogenous human and mouse I kappa B-beta in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse IkB‑ beta Antibody

Detection of Mouse IkB-beta by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, BaF3 mouse pro-B cell line, M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse IkB-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5225) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IkB-beta at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse IkB‑ beta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, BaF3 mouse pro-B cell line, M1 mouse myeloid leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IkB-beta

I kappa B-beta (I kappa B-beta; also NF-kappa-BIB) is a 46 kDa member of the I kappa B-beta inhibitor family of proteins. It is highly expressed in testicular spermatids, and serves to block NF-kappa B activity. This is accomplished by I kappa B-beta binding to either an NF-kappa B dimer, or a kappa B-Ras:NF-kappa B dimer complex. Mouse I kappa B-beta is 359 amino acids (aa) in length it contains a signal response region (aa 1‑50), six ankyrin repeats (aa 54‑308) and a PEST domain whose phosphorylation at Ser312 and Ser314 blocks NF‑ kappa B activation. Phosphorylation at Ser19 and Ser23 promotes I kappa B‑ beta ubiquitination and degradation. Full‑length mouse I kappa B‑ beta shares 94% and 84% aa identity with rat and human I kappa B-beta, respectively.